Department of Oral Biology, Faculty of Dentistry, University of Oslo, Oslo, Norway.
Department of Pathology, B.P. Koirala Memorial Cancer Hospital, Bharatpur, Nepal.
Sci Rep. 2020 May 26;10(1):8737. doi: 10.1038/s41598-020-65694-7.
Helicobacter pylori (HP) infection is an established causative agent for gastric cancer. Although the oral cavity is a part of the gastrointestinal system, the presence and possible causative role of HP in oral squamous cell carcinoma (OSCC) is a subject of controversy. Therefore, the current study aimed to investigate HP infection in two cohorts of OSCC patients with different demographic characteristics, lifestyles and habitual risk factors. A total of 242 formalin-fixed paraffin-embedded OSCC specimens from two different patient cohorts (Norway, n = 171 and Nepal, n = 71) were used to examine HP using immunohistochemistry (IHC) and quantitative polymerase chain reaction (qPCR). Two different HP specific genes (23S rRNA and ureA) were used for TaqMan-based qPCR, and for subsequent verification using HP specific RIDAGENE HP kit and SYBR Green based qPCR. All of the OSCC specimens from both cohorts were found to be negative for HP infection with IHC and qPCR, although the positive control specimens tested positive. Our findings suggest that HP is absent in the examined OSCC cohorts, irrespective of race, lifestyle and habitual risk factors. This indicates that, in contrast to gastric cancer, HP is an unlikely contributing factor for OSCC pathogenesis.
幽门螺杆菌（HP）感染是胃癌的既定致病因素。尽管口腔是胃肠道的一部分，但 HP 在口腔鳞状细胞癌（OSCC）中的存在及其可能的致病作用仍存在争议。因此，本研究旨在调查具有不同人口统计学特征、生活方式和习惯性危险因素的两批 OSCC 患者中 HP 感染情况。使用免疫组织化学（IHC）和实时聚合酶链反应（qPCR），对来自两个不同患者队列（挪威，n=171 和尼泊尔，n=71）的 242 例福尔马林固定石蜡包埋的 OSCC 标本进行了 HP 检测。使用 TaqMan 基于 qPCR 的两种不同的 HP 特异性基因（23S rRNA 和 ureA）进行了检测，并使用 HP 特异性 RIDAGENE HP 试剂盒和基于 SYBR Green 的 qPCR 进行了后续验证。尽管阳性对照标本呈阳性，但两个队列的所有 OSCC 标本均未通过 IHC 和 qPCR 检测到 HP 感染。我们的研究结果表明，在所检查的 OSCC 队列中，无论种族、生活方式和习惯性危险因素如何，HP 均不存在。这表明，与胃癌不同，HP 不太可能是 OSCC 发病机制的一个促成因素。
