人乳头瘤病毒16型E7蛋白人源抗体的检测
Detection of human antibody against the human papillomavirus type 16 E7 protein.
作者信息
Hashido M, Kanda T, Zanma S, Watanabe S, Komiyama N, Yoshikawa H, Yamaguchi N, Kawana T, Yoshiike K
机构信息
Department of Virology, University of Tokyo.
出版信息
Jpn J Cancer Res. 1991 Dec;82(12):1406-12. doi: 10.1111/j.1349-7006.1991.tb01813.x.
Abstract
We examined 500 human sera for the presence of antibody against the human papillomavirus type 16 E7 protein by enzyme-linked immunosorbent assay with bacterially expressed fusion protein lac-E7, and by radioimmunoprecipitation and immunofluorescence assays with the E7 protein expressed in monkey COS-1 cells. The anti-E7 antibody was detectable in 6 out of 54 cases of cervical carcinoma, but in none of the others, including patients with other gynecologic cancers, those with sexually transmitted diseases, and healthy adults. The data indicate that expression of the E7 protein is closely related to cervical carcinoma.
摘要
我们通过用细菌表达的融合蛋白lac-E7进行酶联免疫吸附测定，以及用在猴COS-1细胞中表达的E7蛋白进行放射免疫沉淀和免疫荧光测定，检测了500份人血清中针对人乳头瘤病毒16型E7蛋白的抗体。在54例宫颈癌病例中有6例可检测到抗E7抗体，但在其他病例中均未检测到，包括患有其他妇科癌症的患者、患有性传播疾病的患者和健康成年人。数据表明E7蛋白的表达与宫颈癌密切相关。
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