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本文引用的文献

1
Pre-slaughter handling of reindeer-effects on meat quality.宰前驯鹿处理对肉质的影响。
Meat Sci. 1996;43S1:257-64.
2
Impact of chronic catheterization and automated blood sampling (Accusampler) on serum corticosterone and fecal immunoreactive corticosterone metabolites and immunoglobulin A in male rats.慢性导管插入术和自动采血（Accusampler）对雄性大鼠血清皮质酮、粪便免疫反应性皮质酮代谢物及免疫球蛋白A的影响
J Endocrinol. 2004 Jan;180(1):145-53. doi: 10.1677/joe.0.1800145.
3
Faecal corticosterone and immunoglobulin A in young adult rats.成年幼鼠的粪便皮质酮和免疫球蛋白A
Lab Anim. 2003 Apr;37(2):166-71. doi: 10.1258/00236770360563822.
4
A matter of time: evaluating the storage of fecal samples for steroid analysis.时间问题：评估用于类固醇分析的粪便样本的储存
Gen Comp Endocrinol. 2002 Aug;128(1):57-64. doi: 10.1016/s0016-6480(02)00063-1.
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Development and validation of a sensitive ELISA for quantification of secretory IgA in rat saliva and faeces.
Lab Anim. 2001 Oct;35(4):301-6. doi: 10.1258/0023677011911822.
6
Noninvasive monitoring of adrenocortical activity in roe deer (Capreolus capreolus) by measurement of fecal cortisol metabolites.通过测量粪便皮质醇代谢物对狍（Capreolus capreolus）肾上腺皮质活动进行无创监测。
Gen Comp Endocrinol. 2001 Jul;123(1):111-20. doi: 10.1006/gcen.2001.7656.
7
Are salivary immunoglobulin A and lysozyme biomarkers of stress among nurses?唾液免疫球蛋白A和溶菌酶是护士压力的生物标志物吗？
J Occup Environ Med. 1999 Oct;41(10):920-7. doi: 10.1097/00043764-199910000-00013.
8
Seasonal levels of metabolic hormones and substrates in male and female reindeer (Rangifer tarandus).雄性和雌性驯鹿（Rangifer tarandus）体内代谢激素和底物的季节性水平。
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1998 Aug;120(2):307-15. doi: 10.1016/s0742-8413(98)10010-5.
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Dynamics of stress-related decrease of salivary immunoglobulin A (sIgA): relationship to symptoms of the common cold and studying behavior.
Behav Med. 1998 Winter;23(4):161-9. doi: 10.1080/08964289809596372.
10
Physiological responses and effects on meat quality in reindeer (Rangifer tarandus) transported on lorries.在卡车上运输的驯鹿（Rangifer tarandus）的生理反应及其对肉质的影响。
Acta Vet Scand. 1984;25(1):128-38. doi: 10.1186/BF03547286.

通过对驯鹿血清、唾液、尿液和粪便中的皮质醇、皮质醇免疫反应性代谢物和免疫球蛋白A进行定量分析，以实现对驯鹿应激的无创评估。

Quantification of cortisol, cortisol immunoreactive metabolites, and immunoglobulin A in serum, saliva, urine, and feces for noninvasive assessment of stress in reindeer.

作者信息

Rehbinder Claes, Hau Jann

机构信息

Division of Comparative Medicine, Department of Neuroscience, Uppsala University, Uppsala, Sweden.

出版信息

Can J Vet Res. 2006 Apr;70(2):151-4.

PMID:16639949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1410727/
Abstract

This study was designed to develop reliable methods for quantification of cortisol and cortisol immunoreactive metabolites (C-CIM) and immunoglobulin A (IgA) in reindeer serum, saliva, urine, and feces as tools for the objective noninvasive assessment of well-being and immunocompetence in reindeer. Although C-CIM was readily quantifiable by radioimmunoassay in serum, urine, and feces, the levels in saliva samples were low, rendering quantification unreliable. Whereas IgA concentrations were high in feces samples, they were much lower, albeit quantifiable, in serum and urine; the levels in saliva samples were too low for quantification with the use of an enzyme-linked immunosorbent assay that we developed. Further studies are in progress to validate the usefulness of fecal levels of C-CIM and IgA in the assessment of welfare in reindeer.

摘要

本研究旨在开发可靠的方法，用于定量驯鹿血清、唾液、尿液和粪便中的皮质醇及皮质醇免疫反应性代谢产物（C-CIM）和免疫球蛋白A（IgA），作为客观非侵入性评估驯鹿健康状况和免疫能力的工具。尽管通过放射免疫测定法可轻松定量血清、尿液和粪便中的C-CIM，但唾液样本中的含量较低，使得定量不可靠。虽然粪便样本中IgA浓度较高，但在血清和尿液中要低得多，尽管可定量；唾液样本中的水平过低，无法使用我们开发的酶联免疫吸附测定法进行定量。进一步的研究正在进行中，以验证粪便中C-CIM和IgA水平在评估驯鹿福利方面的有用性。