冲洗牙科用水管路以去除微生物污染物的作用。
The role of flushing dental water lines for the removal of microbial contaminants.
作者信息
Rice Eugene W, Rich William K, Johnson Clifford H, Lye Dennis J
机构信息
U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268, USA.
出版信息
Public Health Rep. 2006 May-Jun;121(3):270-4. doi: 10.1177/003335490612100308.
OBJECTIVES
This study was designed to determine the role of flushing dental water lines for the removal of heterotrophic plate count bacteria, Legionella spp., and free-living protozoa.
METHODS
Forty dental offices were surveyed in the study. An initial sample and a sample taken after three minutes of flushing were obtained from the air/water syringe at each location. All samples were quantitatively analyzed for heterotrophic bacteria using three bacteriological procedures. The samples were analyzed for the presence of Legionella spp. using cultural, immunological, and molecular procedures and for the occurrence of free-living protozoa using a killed bacteria plate procedure.
RESULTS
The flushing process reduced the level of heterotrophic plate count bacteria by 1.1 to 1.5 log10 CFU/ml. Compliance with recommendations for bacterial levels varied depending on the methodology employed in the analysis. The flushing process did not reduce the occurrence of Legionella spp. or free-living protozoa.
CONCLUSION
The results support recent U.S. Centers for Disease Control and Prevention recommendations that the process of flushing dental water lines cannot be relied upon as a sole means of reliably improving the quality of water used in dental treatment.
目的
本研究旨在确定冲洗牙科水线对去除异养平板计数细菌、军团菌属和自由生活原生动物的作用。
方法
本研究对40个牙科诊所进行了调查。从每个地点的空气/水注射器中获取初始样本和冲洗三分钟后的样本。所有样本均采用三种细菌学方法对异养细菌进行定量分析。采用培养、免疫和分子方法分析样本中军团菌属的存在情况，采用死菌平板法分析自由生活原生动物的存在情况。
结果
冲洗过程使异养平板计数细菌水平降低了1.1至1.5 log10 CFU/ml。根据分析所采用的方法，符合细菌水平建议的情况有所不同。冲洗过程并未降低军团菌属或自由生活原生动物的发生率。
结论
结果支持美国疾病控制与预防中心最近的建议，即不能仅依靠冲洗牙科水线的过程来可靠地提高牙科治疗用水的质量。
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