Thompson Lisa, Lindhardt Charlotte
Merck Chemicals Ltd., Newport Technical Centre, 5 The Courtyard, Imperial Park, Newport NP10 8UL, Wales, United Kingdom.
J AOAC Int. 2006 Mar-Apr;89(2):417-32.
Singlepath Salmonella is an immunochromatographic (lateral flow) assay for the presumptive qualitative detection of Salmonella spp. in food. The AOAC Performance-Tested Method study evaluated Singlepath Salmonella as an effective method for the detection of Salmonella spp. in the following selected foods: dried skimmed milk, black pepper, dried pet food, desiccated coconut, cooked peeled frozen prawns, raw ground beef, and raw ground turkey. When the foods were inoculated with Salmonella spp. at levels ranging from low [0.23-1.08 colony forming units (CFU)/25 g] to high (2.3-6.0 CFU/25 g), a Chi-square value of 0.9 indicated that there was no significant difference between Singlepath Salmonella and the ISO 6579:2002 reference method. Singlepath Salmonella gave a false-positive rate of 7.3% and a false-negative rate of 2.5%. For the inclusivity study, all 105 Salmonella serovars reacted with Singlepath Salmonella. For the exclusivity study, 58 non-Salmonella spp. were tested. There were no cross-reactions with Singlepath Salmonella from these strains.
单通道沙门氏菌检测是一种免疫层析（侧向流动）检测方法，用于对食品中的沙门氏菌属进行定性检测。美国官方分析化学师协会（AOAC）性能验证方法研究评估了单通道沙门氏菌检测作为检测以下选定食品中沙门氏菌属的有效方法：脱脂奶粉、黑胡椒、宠物干粮、椰丝、熟去皮冷冻虾、生牛肉末和生火鸡肉末。当食品接种沙门氏菌属的水平从低（0.23 - 1.08菌落形成单位（CFU）/25克）到高（2.3 - 6.0 CFU/25克）时，卡方值为0.9表明单通道沙门氏菌检测与ISO 6579:2002参考方法之间没有显著差异。单通道沙门氏菌检测的假阳性率为7.3%，假阴性率为2.5%。在包容性研究中，所有105种沙门氏菌血清型均与单通道沙门氏菌检测发生反应。在排他性研究中，对58种非沙门氏菌属进行了测试。这些菌株与单通道沙门氏菌检测没有交叉反应。
