Diaz-Torres Martha L, Villedieu Aurelie, Hunt Nigel, McNab Rod, Spratt David A, Allan Elaine, Mullany Peter, Wilson Michael
Division of Microbial Diseases, UCL Eastman Dental Institute, University College London, UK.
FEMS Microbiol Lett. 2006 May;258(2):257-62. doi: 10.1111/j.1574-6968.2006.00221.x.
Studies of the prevalence and identity of genes encoding resistance to antibiotics in a microbial community are usually carried out on only the cultivable members of the community. However, it is possible to include the as-yet-uncultivable organisms present by adopting a metagenomic approach to such studies. In this investigation, four metagenomic libraries of the oral microbiota were prepared from three groups of 20 adult humans and screened for antibiotic-resistant clones. Clones resistant to tetracycline and amoxycillin were present in all four libraries while gentamicin-resistant clones were found in three of the libraries. The genes encoding tetracycline resistance in the clones were identified and found to be tet(M), tet(O), tet(Q), tet(W), tet37 and tet(A). However, only the first three of these were detected in all three groups of individuals investigated.
对微生物群落中编码抗生素抗性的基因的流行情况和特性的研究通常仅针对群落中可培养的成员进行。然而，通过在这类研究中采用宏基因组学方法，有可能将目前尚未培养的生物体纳入其中。在本研究中，从三组20名成年人中制备了口腔微生物群的四个宏基因组文库，并筛选了抗抗生素克隆。在所有四个文库中均存在对四环素和阿莫西林耐药的克隆，而在其中三个文库中发现了对庆大霉素耐药的克隆。对克隆中编码四环素抗性的基因进行了鉴定，发现是tet(M)、tet(O)、tet(Q)、tet(W)、tet37和tet(A)。然而，在所有三组被调查个体中仅检测到其中前三个基因。
