Stipcevic Tamara, Piljac Jasenka, Vanden Berghe Dirk
Department of Molecular Medicine, Rudjer Boskovic Institute, Zagreb, Croatia.
Plant Foods Hum Nutr. 2006 Mar;61(1):29-34. doi: 10.1007/s11130-006-0006-8.
In this study, we discovered that flavonoids belonging to the subclasses: (flavanone, flavone, and flavonol) display differential effects on the synthesis of collagen in human dermal fibroblasts. At 80 microg/ml flavonoids quercetin-3,3',4', 5,7-pentahydroxyflavone, 3-methyl quercetin, and 7-hydroxyflavone significantly decreased the total protein concentration which was a direct consequence of their cytotoxic effect, while naringenin exhibited no effect on total collagen and total protein concentration. Quercetin-3,3'4',7-tetramethyl ether, 4'-hydroxyflavanone, flavanone, and fisetin significantly decreased collagen concentration while morin, rutin, and chrysin increased collagen concentration without changing the overall protein concentration. The initial screening performed in this study enables the identification of compounds that exert significant effects on fibroblast function and show potential as starting material for pharmaceutical preparations targeted against various disorders centered around disturbed collagen metabolism.
在本研究中，我们发现属于以下亚类的黄酮类化合物（黄烷酮、黄酮和黄酮醇）对人皮肤成纤维细胞中胶原蛋白的合成具有不同的影响。在80微克/毫升的黄酮类化合物中，槲皮素-3,3',4',5,7-五羟基黄酮、3-甲基槲皮素和7-羟基黄酮显著降低了总蛋白浓度，这是它们细胞毒性作用的直接结果，而柚皮苷对总胶原蛋白和总蛋白浓度没有影响。槲皮素-3,3',4',7-四甲醚、4'-羟基黄烷酮、黄烷酮和漆黄素显著降低了胶原蛋白浓度，而桑色素、芦丁和白杨素增加了胶原蛋白浓度，同时总体蛋白浓度没有变化。本研究中进行的初步筛选能够鉴定出对成纤维细胞功能有显著影响且有潜力作为针对各种以紊乱的胶原蛋白代谢为中心的疾病的药物制剂起始原料的化合物。
