An Bong-Jeun, Kwak Jae-Hoon, Park Jung-Mi, Lee Jin-Young, Park Tae-Soon, Lee Jin-Tae, Son Jun-Ho, Jo Cheorun, Byun Myung-Woo
Department of Cosmeceutical Sciene, Daegu Haany University, 712-715 Kyungsan, South Korea.
Dermatol Surg. 2005 Jul;31(7 Pt 2):848-54; discussion 854. doi: 10.1111/j.1524-4725.2005.31730.
Many studies have been conducted to find a natural material that has high biologic functions for human skin without any side effects. Persimmon leaf has a substantial amount of tannins in different forms; therefore, it was selected as a target material.
The biosynthesis rate of the collagen was also investigated to clarify the beneficial functions for the human skin.
Persimmon leaves were obtained, extracted with 80% ethanol, and isolated into PFs I, II, and III after column chromatography using a Sephadex LH-20 column followed by thin-layer chromatography.
The xanthine oxidase inhibition effect of both PFs II and III was over 40% at a 100 ppm concentration. PF II, containing higher flavonoids levels, had a significantly higher tyrosinase inhibition than that of PF III. Collagenase inhibition was 16.3 and 8.1% for PF III and PF II, respectively, at 100 ppm. On the other hand, elastase inhibition activity was significantly higher in PF II than PF III. Collagen biosynthesis rates of PF III were over 25% from a 1 to 10 ppm concentration. Consequently, PFs isolated from the persimmon leaf can be used as natural materials or additives for human skin owing to their beneficial biologic functions, including the antiwrinkle effect and the inhibition of skin problems, for food or cosmetic compositions.
已经进行了许多研究以寻找一种对人体皮肤具有高生物学功能且无任何副作用的天然材料。柿叶含有大量不同形式的单宁；因此，它被选为目标材料。
还研究了胶原蛋白的生物合成率，以阐明其对人体皮肤的有益功能。
获取柿叶，用80%乙醇提取，然后使用葡聚糖LH-20柱进行柱色谱，再经薄层色谱分离得到PFs I、II和III。
在100 ppm浓度下，PFs II和III的黄嘌呤氧化酶抑制作用均超过40%。PF II含有较高水平的黄酮类化合物，其酪氨酸酶抑制作用明显高于PF III。在100 ppm时，PF III和PF II的胶原酶抑制率分别为16.3%和8.1%。另一方面，PF II的弹性蛋白酶抑制活性明显高于PF III。从1到10 ppm浓度，PF III的胶原蛋白生物合成率超过25%。因此，从柿叶中分离出的PFs因其有益的生物学功能，包括抗皱作用和对皮肤问题的抑制作用，可作为食品或化妆品组合物中用于人体皮肤的天然材料或添加剂。
