Yang Wei-Cheng, Pang Victor Fei, Jeng Chian-Ren, Chou Lien-Siang, Chueh Ling-Ling
Graduate Institute of Veterinary Medicine, National Taiwan University, Taipei 106, Taiwan, ROC.
Vet Microbiol. 2006 Aug 25;116(1-3):69-76. doi: 10.1016/j.vetmic.2006.03.014. Epub 2006 Apr 27.
Morbilliviral infection was diagnosed in an adult male pygmy sperm whale (Kogia breviceps) from southwestern Taiwan on the basis of pathological findings, immunohistochemical staining, and reverse transcription-polymerase chain reaction. The whale was found alive stranded on the beach and died after 5 days of medical care. It was thin and had dozens of nematode in the first stomach. The lungs were dark red and heavy. Histopathological examination revealed diffuse, moderate bronchointerstitial pneumonia. Intranuclear and intracytoplasmic inclusions with occasional syncytial cell formation were noted in the lungs, lymph nodes, and spleen. The RNA extracted from lung tissue was subjected to morbilliviral gene amplification. After priming with specific oligonucleotides, the cDNA covering the phosphoprotein (P) gene was copied and then amplified by PCR. The gene fragment amplified from the lung tissue was sequenced. Phylogenetic analysis of partial P gene revealed 97.6% sequence identity to the dolphin morbillivirus and 90.2% similarity to the pilot whale morbillivirus. Morbilliviral antigens were detected in the lungs, lymph nodes, and spleen by immunohistochemistry using polyclonal antibody against rinderpest virus. This is the first report of morbilliviral infection with genetic evidence in a pygmy sperm whale from the Western Pacific Ocean around Taiwan.
根据病理检查结果、免疫组织化学染色及逆转录聚合酶链反应，在一头来自台湾西南部的成年雄性侏儒抹香鲸（小抹香鲸）身上诊断出麻疹病毒感染。这头鲸鱼被发现时活着搁浅在海滩上，在接受5天医疗护理后死亡。它很瘦，第一胃中有几十条线虫。肺呈暗红色且沉重。组织病理学检查显示为弥漫性中度支气管间质性肺炎。在肺、淋巴结和脾脏中发现了核内和胞浆内包涵体，偶尔可见多核巨细胞形成。从肺组织中提取的RNA进行了麻疹病毒基因扩增。用特异性寡核苷酸引物后，覆盖磷蛋白（P）基因的cDNA被复制，然后通过PCR扩增。对从肺组织扩增的基因片段进行测序。部分P基因的系统发育分析显示与海豚麻疹病毒的序列同一性为97.6%，与领航鲸麻疹病毒的相似性为90.2%。使用抗牛瘟病毒的多克隆抗体通过免疫组织化学在肺、淋巴结和脾脏中检测到麻疹病毒抗原。这是台湾附近西太平洋地区侏儒抹香鲸感染麻疹病毒并具有基因证据的首次报告。
