Scarpelli Daniela, Cardellini Marina, Andreozzi Francesco, Laratta Emanuela, Hribal Marta Letizia, Marini Maria Adelaide, Tassi Vittorio, Lauro Renato, Perticone Francesco, Sesti Giorgio
Dipartimento di Medicina Sperimentale e Clinica, Policlinico Mater Domini, University Magna Graecia of Catanzaro, Via Tommaso Campanella, 88100 Catanzaro, Italy.
Diabetes. 2006 May;55(5):1529-33. doi: 10.2337/db06-0047.
Interleukin (IL)-10 is a major anti-inflammatory cytokine that has been associated with obesity and type 2 diabetes. The three polymorphisms -1082G/A, -819C/T, and -592C/A in the IL10 promoter were reported to influence IL10 transcription. We investigated whether these polymorphisms were associated with type 2 diabetes and related traits in a cohort of Italian Caucasians comprising 551 type 2 diabetic and 1,131 control subjects. The -819C/T and -592C/A polymorphisms were in perfect linkage disequilibrium (r(2) = 1.0). The -1082G/A polymorphism was not associated with type 2 diabetes or related traits. Although the -592C/A polymorphism was not associated with type 2 diabetes, nondiabetic homozygous carriers of the A allele showed increased BMI and insulin resistance and lower plasma IL-10 levels compared with the other genotypes. In the nondiabetic group, the ATA haplotype was associated with an increased risk for obesity (odds ratio 1.28 [95% CI 1.02-1.60]; P = 0.02). The ATA/ATA composite genotype was associated with an increased risk for obesity (1.96 [1.16-3.31]; P = 0.01) and insulin resistance (1.99 [1.12-3.53]; P = 0.01). This study suggests that polymorphisms and haplotypes of the IL10 promoter may be associated with obesity and insulin resistance in a large sample of Italian Caucasians.
白细胞介素（IL）-10是一种主要的抗炎细胞因子，与肥胖症和2型糖尿病有关。据报道，IL10启动子中的三种多态性——-1082G/A、-819C/T和-592C/A会影响IL10的转录。我们在一个由551名2型糖尿病患者和1131名对照受试者组成的意大利白种人队列中，研究了这些多态性是否与2型糖尿病及相关特征有关。-819C/T和-592C/A多态性处于完全连锁不平衡状态（r(2)=1.0）。-1082G/A多态性与2型糖尿病或相关特征无关。虽然-592C/A多态性与2型糖尿病无关，但与其他基因型相比，A等位基因的非糖尿病纯合携带者的BMI和胰岛素抵抗增加，血浆IL-10水平降低。在非糖尿病组中，ATA单倍型与肥胖风险增加有关（优势比1.28[95%CI 1.02-1.60]；P=0.02）。ATA/ATA复合基因型与肥胖风险增加（1.96[1.16-3.3l]；P=0.01）和胰岛素抵抗增加（1.99[1.12-3.53]；P=0.01）有关。这项研究表明，在大量意大利白种人样本中，IL10启动子的多态性和单倍型可能与肥胖症和胰岛素抵抗有关。
