Li Li, Mac-Mary Sophie, Sainthillier Jean-Marie, Nouveau Stephanie, de Lacharriere Olivier, Humbert Philippe
Cutaneous Engineering and Biology Laboratory and IFR 133 Cell and Tissue Engineering and Biology, University of Franche-Comté, Besançon, France.
Gerontology. 2006;52(3):142-53. doi: 10.1159/000091823.
The architectural frameworks of the skin microcirculation are rather complex and change continuously with aging. But these changes are yet poorly documented in vivo.
Using non-invasive methods belonging to the field of biometrology, the study aimed to investigate quantitatively the changes of the cutaneous microvasculature in different anatomic sites with age.
Measurements were performed on crow's feet, forehead, volar forearm and dorsum of hand in 50 women (aged 20-74 years who consisted of 10 probands in each live decades). The superficial vascular plexus was scanned by videocapillaroscopy and assessed with the software Capilab Toolbox. The subpapillary vascular plexus was explored with laser Doppler flowmetry. The skin color a* was analyzed by chromametry.
A marked site and age effect on the skin microcirculation has been demonstrated. The density of capillary loops in the eldest group decreased by about 40-70% compared with the youngest group whereas the vascular length increased by 35-156%. The capillary density in the back of the hand was 4 times higher than in the crow's feet. The vascular length in the crow's feet was 3 times longer than in the back of the hand. Both blood flow and skin redness (a*) increased also with age.
Both morphology and quantification of the cutaneous microvasculature showed changes with site and age. Videocapillaroscopy associated to an image processing and laser Doppler flowmetry revealed different vascular layers. So the combination of both instruments offers an easy way to observe the architectural frameworks in vivo.
皮肤微循环的结构框架相当复杂，且会随着衰老不断变化。但这些变化在体内的记录仍很少。
本研究旨在利用生物计量学领域的非侵入性方法，定量研究不同解剖部位的皮肤微血管系统随年龄的变化。
对50名女性（年龄20 - 74岁，每十年年龄组有10名受试者）的鱼尾纹、前额、前臂掌侧和手背进行测量。通过视频毛细血管显微镜扫描浅表血管丛，并用Capilab Toolbox软件进行评估。用激光多普勒血流仪探测乳头下血管丛。通过色度法分析皮肤颜色a*。
已证实皮肤微循环存在显著的部位和年龄效应。与最年轻组相比，最年长组的毛细血管袢密度降低了约40 - 70%，而血管长度增加了35 - 156%。手背的毛细血管密度比鱼尾纹处高4倍。鱼尾纹处的血管长度比手背长3倍。血流量和皮肤发红程度（a*）也随年龄增加。
皮肤微血管系统的形态和量化均显示出随部位和年龄的变化。视频毛细血管显微镜结合图像处理和激光多普勒血流仪揭示了不同的血管层。因此，这两种仪器的组合提供了一种在体内观察结构框架的简便方法。
