Ishiyama J, Saito H, Abe K
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Neurosci Res. 1991 Nov;12(3):403-11. doi: 10.1016/0168-0102(91)90071-6.
The effects of epidermal growth factor (EGF), basic fibroblast growth factor (bFGF) and nerve growth factor (NGF) on long-term potentiation (LTP) of evoked potential were investigated in the dentate gyrus of anaesthetised rats. Tetanic stimulation of 100 pulses at 100 Hz was applied to induce complete LTP, and stimulation of 20 pulses at 60 Hz was used as a subthreshold stimulation in inducing LTP. Injection of 50 ng EGF, bFGF or NGF into the contralateral ventricle influenced neither the basal amplitude of the population spike nor the LTP induced by the tetanus of 100 pulses at 100 Hz. However, EGF or bFGF, but not NGF, significantly augmented the potentiation induced by the tetanus of 20 pulses at 60 Hz and facilitated the generation of LTP. Moreover, the effect of EGF was dose-dependent in the range 5-500 ng. These results suggest that EGF and bFGF promote the generation process of LTP.
在麻醉大鼠的齿状回中，研究了表皮生长因子（EGF）、碱性成纤维细胞生长因子（bFGF）和神经生长因子（NGF）对诱发电位长时程增强（LTP）的影响。采用100Hz的100次脉冲强直刺激诱导完全LTP，60Hz的20次脉冲刺激作为诱导LTP的阈下刺激。向对侧脑室注射50ng EGF、bFGF或NGF，既不影响群体峰电位的基础幅度，也不影响100Hz的100次脉冲强直刺激诱导的LTP。然而，EGF或bFGF（而非NGF）显著增强了60Hz的20次脉冲强直刺激诱导的增强效应，并促进了LTP的产生。此外，EGF在5-500ng范围内的作用呈剂量依赖性。这些结果表明，EGF和bFGF促进了LTP的产生过程。
