Department of Horticulture, Purdue University, Lafayette, Indiana.
Plant Physiol. 1967 Dec;42(12):1673-9. doi: 10.1104/pp.42.12.1673.
The light stimulation of cold acclimation of Hedera helix L. var. Thorndale has been shown to result in the production of translocatable promoters of hardiness. Movement of the promoters from an illuminated donor portion to a darkened receptor portion was demonstrated. The majority of transport was acropetal through the phloem and to a much lesser degree basipetal through the xylem. In the early stages of hardening, transport was strictly acropetal. It is suggested that acropetal transport is under the influence of a mobilizing center located in the apex of the plant. Mobilization of the promoters of hardiness was induced by applications of (6)N-benzyladenine. Attempts to characterize the light-generated promoters through fixation of (14)CO(2) and subsequent translocation of (14)C-labeled compounds from the illuminated donor to the darkened receptor indicated that the translocatable promoting material was either some component of the Dowex 1 fraction or sucrose. Furthermore, the hardiness of leaves was significantly increased by sucrose solutions but not by equi-molar solutions of glucose, galactose or mannitol.
低温驯化的常春藤的光刺激已被证明会导致耐寒性的可移动启动子的产生。已经证明启动子从受光照的供体部分向黑暗的受体部分移动。大部分运输是通过韧皮部向顶运动，通过木质部向基运动的程度要小得多。在硬化的早期阶段，运输是严格的向顶运动。有人认为，向顶运输受位于植物顶端的动员中心的影响。用（6）N-苄基腺嘌呤处理可以诱导耐寒性启动子的动员。通过固定（14）CO（2）并随后将（14）C 标记化合物从受光照的供体转移到黑暗的受体，试图对光产生的启动子进行特征分析，表明可转移的促进物质要么是 Dowex 1 级分的某种成分，要么是蔗糖。此外，蔗糖溶液显著增加了叶片的耐寒性，但等摩尔浓度的葡萄糖、半乳糖或甘露醇溶液则没有。
