Department of Agronomy, University of Kentucky, Lexington, Kentucky 40506.
Plant Physiol. 1967 Dec;42(12):1731-6. doi: 10.1104/pp.42.12.1731.
Excised roots of barley (Hordeum vulgare, var. Campana) lost organic acids, amino acids, K(+), and Cl(-) within 15 minutes after initiation of anaerobic treatment or treatment with NaCN and 2,4-dinitrophenol. Initial loss of organic acids when roots were placed under N(2) is attributed to a decarboxylation reaction, possibly catalyzed by phosphoenolpyruvate carboxykinase. Organic and amino acids began to leak from the roots to the bathing medium after 1 to 2 hours under N(2), indicating injury to cell membranes. During the first hour of anaerobic treatment, K(+) loss from low-salt roots was equivalent to organic acid loss. Potassium loss from roots containing high levels of KCl was approximately equal to organic acid plus amino acid loss; and Cl(-) loss was approximately equal to amino acid loss. It is postulated that, within cells, organic acids may electrostatically bind an equivalent quantity of cations and that amino acids may bind an equivalent quantity of both cations and anions.
大麦（Hordeum vulgare，var. Campana）的离体根在厌氧处理或用 NaCN 和 2,4-二硝基苯酚处理后 15 分钟内失去有机酸、氨基酸、K(+)和 Cl(-)。当根在 N(2)下放置时，有机酸的初始损失归因于脱羧反应，可能由磷酸烯醇丙酮酸羧激酶催化。在 N(2)下 1 至 2 小时后，有机和氨基酸开始从根渗出到浴液中，表明细胞膜受到损伤。在厌氧处理的第一个小时内，低盐根中 K(+)的损失与有机酸的损失相当。含高浓度 KCl 的根中 K(+)的损失约等于有机酸加氨基酸的损失；Cl(-)的损失与氨基酸的损失大致相等。据推测，在细胞内，有机酸可能通过静电作用与等量的阳离子结合，而氨基酸可能与等量的阳离子和阴离子结合。
