组织培养物冠瘿中细胞分裂素的色谱分析。
Chromatographic analysis of a cytokinin from tissue cultures of crown-gall.
机构信息
Department of Biology, The Pennsylvania State University, University Park, Pennsylvania 16802.
出版信息
Plant Physiol. 1971 Apr;47(4):581-5. doi: 10.1104/pp.47.4.581.
Abstract
Extracts from tissue cultures of crown-gall from Parthenocissus tricuspidata (Sieb. and Zucc.) Planch. exhibited cell division activity in the soybean cytokinin assay. The chromatographic migration of one component, responsible for most of the activity, is similar to that of zeatin ribonucleoside. In addition, acid hydrolysis of the active region taken from chromatograms and of an eluate from a cation-exchange resin column resulted in the production of an active free-base-like derivative. Since the derivative and the parent compound lose activity after KMnO(4) treatment, they are believed to possess an unsaturated constituent essential for biological activity. The major active factor present in crown-gall from Parthenocissus tricuspidata is therefore distinct from the nicotinamide derivative reported to be present in Vinca rosea L. tumor cells.
摘要
从五叶地锦的根癌组织培养物中提取的物质，在大豆细胞分裂素测定中显示出细胞分裂活性。一种主要负责大部分活性的成分的色谱迁移与玉米素核苷相似。此外，从色谱图中的活性区域和阳离子交换树脂柱洗脱液中进行的酸水解导致产生一种具有活性的游离碱基类似物。由于衍生化合物和母体化合物在 KMnO4 处理后失去活性，因此它们被认为具有生物活性所必需的不饱和成分。因此，五叶地锦根癌中存在的主要活性因子与报道存在于长春花肿瘤细胞中的烟酰胺衍生物不同。
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