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萌发小麦胚中脱氧核糖核酸合成的起始

Onset of deoxyribonucleic Acid synthesis in germinating wheat embryos.

作者信息

Mory Y Y, Chen D, Sarid S

机构信息

Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.

出版信息

Plant Physiol. 1972 Jan;49(1):20-3. doi: 10.1104/pp.49.1.20.

Abstract

Germinating wheat embryos (Triticum vulgare var. Florence) synthesize proteins before the onset of DNA synthesis. The onset of DNA replication occurs at about 15 hours of germination and was shown to depend on proteins synthesized before 9 hours of germination with the use of blasticidin S, a specific inhibitor of protein synthesis. A 10-fold increase in the activity of DNA-dependent DNA polymerase was found in extracts derived from germinated embryos, as compared to the activity found in extracts from ungerminated embryos.

摘要

萌发的小麦胚(普通小麦变种佛罗伦萨)在DNA合成开始之前就合成蛋白质。DNA复制的开始发生在萌发约15小时时,并且通过使用蛋白质合成的特异性抑制剂杀稻瘟菌素S表明,它依赖于萌发9小时之前合成的蛋白质。与未萌发胚提取物中的活性相比,在萌发胚提取物中发现依赖于DNA的DNA聚合酶的活性增加了10倍。

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