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影响小麦胚萌发过程中脱氧核糖核酸合成起始的因素。DNA聚合酶A、B和C以及DNA前体库变化的研究。

Factors affecting the onset of deoxyribonucleic acid synthesis during wheat embryo germination. Study of the changes in DNA polymerases A, B and C and the pool of DNA precursors.

作者信息

Castroviejo M, Tharaud D, Mocquot B, Litvak S

出版信息

Biochem J. 1979 Jul 1;181(1):193-9. doi: 10.1042/bj1810193.

Abstract

DNA synthesis starts about 12 h after water imbibition in wheat embryos. We have determined that noticeable amounts of labelled thymidine are found inside the embryo only after 6 hr of germination. DNA polymerase C from ungerminated wheat embryos decreased markedly in activity during the first hours of germination, whereas the activities of DNA polymerases A and B increased, having a maximum at about 15 h or germination. Serological evidence has suggested a clear antigenic relationship between DNA polymerases A and C. Although the pool of ATP increases rapidly after water imbibition, the increase in the pool of dNTP species was much slower.

摘要

小麦胚吸水后约12小时开始DNA合成。我们已经确定,只有在萌发6小时后,才能在胚内发现大量标记的胸腺嘧啶核苷。未萌发小麦胚中的DNA聚合酶C在萌发的最初几个小时内活性显著下降,而DNA聚合酶A和B的活性增加,在萌发约15小时时达到最大值。血清学证据表明DNA聚合酶A和C之间存在明显的抗原关系。虽然吸水后ATP库迅速增加,但dNTP种类库的增加要慢得多。

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