Department of Biophysics, The Weizmann Institute of Science, Rehovot, Israel.
Plant Physiol. 1974 Mar;53(3):377-81. doi: 10.1104/pp.53.3.377.
A soluble DNA-dependent DNA polymerase was extracted from wheat embryos. In vitro, the incorporation of radioactive thymidine triphosphate into acid-insoluble material is dependent upon the presence of the enzyme, all four deoxyribonucleotide triphosphates, Mg(2+), and a DNA template. Incorporation occurs on native, alkali-denatured, and strictly double-stranded DNA. The in vitro synthesized product is a polydeoxynucleotide with a chain length shorter than the template; it has the same buoyant density as wheat embryo DNA when this DNA is used as template; and it forms a double-stranded complex with the DNA template. These data suggest that the in vitro DNA synthesis catalyzed by proteins extracted from wheat embryos occurs in a semiconservative way.
从小麦胚中提取出一种可溶的 DNA 依赖的 DNA 聚合酶。在体外，放射性胸腺嘧啶三磷酸掺入酸不溶性物质的过程依赖于酶、四种脱氧核苷酸三磷酸、Mg(2+)和 DNA 模板的存在。掺入发生在天然、碱变性和严格的双链 DNA 上。体外合成的产物是一种聚脱氧核苷酸，其链长短于模板；当使用这种 DNA 作为模板时，它具有与小麦胚 DNA 相同的浮力密度；并且它与 DNA 模板形成双链复合物。这些数据表明，从小麦胚中提取的蛋白质催化的体外 DNA 合成是以半保守方式进行的。
