Department of Botany and Plant Pathology, University of Maine at Orono, Orono, Maine 04473.
Plant Physiol. 1973 May;51(5):967-72. doi: 10.1104/pp.51.5.967.
Linear increments in ferulic acid concentration produce logarithmic increases in the ferulic acid-induced lag periods prior to the peroxidase-catalyzed oxidation of indole-3-acetic acid in a system containing 2,4-dichlorophenol and MnCl(2) in acetate buffer at pH 5.6. Maintaining the ratio of indole-3-acetic acid to ferulic acid constant at 100 while linearly raising the ferulic acid concentration results in linear increases in the lag period. Both indole-3-acetic acid and ferulic acid are substrates of horseradish peroxidase in the presence of H(2)O(2), and indole-3-acetic acid competitively inhibits the oxidation of ferulic acid. A model for the enzymatic oxidation of indole-3-acetic acid catalyzed by peroxidase is proposed.
在线性增加阿魏酸浓度的情况下，在含有 2,4-二氯苯酚和 MnCl2 的醋酸缓冲液中，在 pH 5.6 下，过氧化物酶催化吲哚-3-乙酸氧化之前，阿魏酸诱导的滞后时间会呈对数增加。当保持吲哚-3-乙酸与阿魏酸的比例恒定时为 100，而线性提高阿魏酸浓度时，滞后时间会呈线性增加。在 H2O2 的存在下，吲哚-3-乙酸和阿魏酸都是辣根过氧化物酶的底物，并且吲哚-3-乙酸竞争性地抑制阿魏酸的氧化。提出了一种过氧化物酶催化吲哚-3-乙酸氧化的酶促氧化模型。
