蓝光诱导的玉米和 Neurospora 膜片段的吸光度变化。
Blue light-induced Absorbance Changes in Membrane Fractions from Corn and Neurospora.
机构信息
Department of Plant Biology, Carnegie Institution of Washington, Stanford, California 94305.
出版信息
Plant Physiol. 1977 May;59(5):948-52. doi: 10.1104/pp.59.5.948.
Blue light-induced absorbance changes were measured from differentially centrifuged membrane fractions from dark-grown coleoptiles of Zea mays L., and mycelia from an albino mutant of Neurospora crassa. Actinic irradiation caused changes in absorbance consistent with a flavinmediated reduction of a b-type cytochrome. Both corn and Neurospora showed similar light-minus-dark difference spectra, dose response curves, and kinetics of dark recovery after irradiation. The photoreducible cytochrome system from Neurospora showed the same distribution as the activity of a sodium-stimulated adenosine triphosphatase, thought to be a plasma membrane marker, in differential centrifugation experiments. The fraction showing the absorbance change did not co-sediment with the mitochondria, nor with the endoplasmic reticulum. Comparison of absorption spectra of fully oxidized, partially reduced, and fully reduced preparations showed that approximately a 30% reduction of the cytochromes involved with the process was needed to obtain the light-induced absorbance changes.
蓝光诱导的吸收变化从玉米暗生长的 coleoptiles 的差异离心膜部分和 Neurospora crassa 的白化突变体的菌丝体中进行了测量。光照射引起的吸收变化与黄素介导的 b 型细胞色素还原一致。玉米和 Neurospora 都表现出相似的光暗差光谱、剂量响应曲线和光照射后暗恢复的动力学。来自 Neurospora 的光还原细胞色素系统在差速离心实验中与被认为是质膜标记物的钠离子刺激的三磷酸腺苷酶的活性具有相同的分布。显示吸收变化的部分与线粒体或内质网都不共沉淀。完全氧化、部分还原和完全还原制剂的吸收光谱比较表明，该过程中涉及的细胞色素需要大约 30%的还原才能获得光诱导的吸收变化。
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