Department of Horticulture, Zhejiang Agricultural University, 310029, Hangzhou, People's Republic of China.
Planta. 1989 Dec;180(1):131-3. doi: 10.1007/BF02411419.
Leaf explants of 24 cultivars and 2 F1 hybrids of the common tomato (Lycopersicon esculentum Mill.) and ofL. pimpinellifolium Brezh. were cultured on Murashige-Skoog medium containing different concentrations of NaCl. The cultures of 11 genotypes formed flower buds when cultured on medium containing 0.5% NaCl. Flower formation occurred either by direct differentiation from the leaf cultures or by transition of the apices of regenerated shoots from the vegetative state to floral buds. No flower formation occurred on medium without NaCl or media with 1.0% NaCl or more. There existed great differences in the capacity of in-vitro flower formation in the tomato leaf explants among the genotypes tested. The genotypes whose explants did form flowers were all of determinate growth habit.
24 个栽培品种和 2 个普通番茄(Lycopersicon esculentum Mill.)和 L. pimpinellifolium Brezh. 的 F1 杂种的叶片外植体在含有不同浓度 NaCl 的 Murashige-Skoog 培养基上培养。11 种基因型的培养物在含有 0.5%NaCl 的培养基上培养时形成花芽。花芽的形成要么是通过叶片培养物的直接分化,要么是通过再生芽的顶端从营养状态向花芽的过渡。在没有 NaCl 的培养基或含有 1.0%NaCl 或更高浓度的培养基上均未形成花芽。在测试的基因型中,番茄叶片外植体的体外花形成能力存在很大差异。那些外植体能够形成花的基因型均为有限生长习性。