Central Research and Development Department, Experimental Station, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898.
Plant Physiol. 1979 Apr;63(4):744-8. doi: 10.1104/pp.63.4.744.
p-Chloromercuribenzenesulfonic acid markedly inhibited sucrose accumulation into sugar beet source leaves without inhibiting hexose accumulation. The site of inhibition is proposed to be the plasmalemma ATPase, since the ATPase-mediated H(+) efflux was completely inhibited by p-chloromercuribenzenesulfonic acid under conditions where intracellular metabolism, as measured by photosynthesis and hexose accumulation, was unaffected. Fusicoccin, a potent activator of active H(+)/K(+) exchange, stimulated both active sucrose accumulation and proton efflux in the sugar beet leaf tissue. These data provide strong evidence for the phloem loading of sucrose being coupled to a proton transport mechanism driven by a vectorial plasmalemma ATPase.
对氯汞苯磺酸显著抑制了蔗糖在甜菜源叶中的积累，而不抑制己糖的积累。抑制的部位被提议是质膜 ATP 酶，因为在质膜 ATP 酶介导的 H+外排过程中，当细胞内代谢（如光合作用和己糖积累）不受影响时，对氯汞苯磺酸完全抑制了 H+的外排。 Fusicoccin，一种有效的活性 H+/K+交换激活剂，刺激了甜菜叶片组织中活性蔗糖的积累和质子的外排。这些数据为韧皮部装载蔗糖与质子运输机制偶联提供了有力的证据，质子运输机制由向量质膜 ATP 酶驱动。
