Post Harvest Plant Physiology Laboratory and Cell Culture and Nitrogen Fixation Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture, Beltsville, Maryland 20705.
Plant Physiol. 1979 May;63(5):811-5. doi: 10.1104/pp.63.5.811.
Cortex tissue from postclimacteric ;Golden Delicious' apples (Malus domestica, Borkh.) stored at 0 C for 9 months after harvest were induced to form callus in vitro. Cell suspension cultures were subsequently formed from calli. Of five media tested, only the medium of Schenk and Hildebrandt (Can J Bot 1972, 50: 192) and that of Uchimiya and Murashige (Plant Physiol 1974, 54: 936) allowed callus formation. During growth both the callus and cell cultures produced ethylene in a pattern which showed a rapid rise and then a fall as the culture grew. (14)C-Labeled methionine was converted to labeled ethylene by the cell suspension cultures, which also could be inhibited from producing ethylene by a rhizobitoxine analog or free radical scavengers. Ethylene production in these cultures, like that in intact fruit tissue slices, could be stimulated by IAA or suppressed by N(6)-(gamma,gamma-dimethylallyl) adenosine and GA(3).
采后 9 个月在 0°C 下贮藏的后熟期“金冠”苹果（Malus domestica，Borkh.）皮层组织在离体条件下诱导愈伤组织形成。随后从愈伤组织中形成细胞悬浮培养物。在测试的五种培养基中，只有 Schenk 和 Hildebrandt（Can J Bot 1972，50：192）和 Uchimiya 和 Murashige（Plant Physiol 1974，54：936）的培养基允许愈伤组织形成。在生长过程中，愈伤组织和细胞培养物均以快速上升然后下降的模式产生乙烯，随着培养物的生长。（14）C-标记的蛋氨酸被细胞悬浮培养物转化为标记的乙烯，根瘤菌素类似物或自由基清除剂也可以抑制其产生乙烯。这些培养物中的乙烯生成，就像完整的果实组织切片中的乙烯生成一样，可以被 IAA 刺激或被 N（6）-（γ，γ-二甲基丙烯基）腺苷和 GA（3）抑制。
