Prairie Regional Laboratory, National Research Council of Canada, Saskatoon, Saskatchewan, Canada.
Plant Physiol. 1971 Oct;48(4):399-401. doi: 10.1104/pp.48.4.399.
Cell suspension cultures of Ruta graveolens (rue) and Rosa sp. produce ethylene. Both cultures grow at a high rate in hormone-free media. The rose cells are undifferentiated while the Ruta cells differentiate and form shoots after extended culture in hormone-free medium. Addition of 2,4-dichlorophenoxyacetic acid stimulated ethylene production in Ruta cells but not in rose cells. Abscisic acid (ABA) inhibited growth and ethylene production in rose, but only ethylene production in Ruta cells. Addition of kinetin reversed the inhibition by abscisic acid in the rose cells but not in the Ruta cells. The results suggested a distinct physiological difference between the two cultures. The Ruta cells responded to the growth regulators in a manner similar to whole plants.
瑞香狼毒（ Rue ）和蔷薇属（ Rosa ）细胞悬浮培养物会产生乙烯。这两种培养物在无激素培养基中均能以较高的速率生长。蔷薇属细胞为未分化状态，而瑞香狼毒细胞在无激素培养基中长时间培养后会分化并形成芽。2,4-二氯苯氧乙酸的添加会刺激瑞香狼毒细胞产生乙烯，但不会刺激蔷薇属细胞。脱落酸（ABA）抑制了蔷薇属细胞的生长和乙烯产生，但仅抑制了瑞香狼毒细胞的乙烯产生。添加激动素会逆转 ABA 对蔷薇属细胞的抑制，但对瑞香狼毒细胞无影响。这些结果表明两种培养物之间存在明显的生理差异。瑞香狼毒细胞对生长调节剂的反应方式与整株植物相似。
