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与纯化的核酮糖二磷酸羧化酶相关的内肽酶活性的来源。

Source of endoproteolytic activity associated with purified ribulose bisphosphate carboxylase.

机构信息

Plant Growth Laboratory and the Department of Agronomy and Range Science, University of California, Davis, California 95616.

出版信息

Plant Physiol. 1984 May;75(1):74-7. doi: 10.1104/pp.75.1.74.

DOI:10.1104/pp.75.1.74
PMID:16663605
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1066837/
Abstract

The association of endoproteolytic activity with purified ribulose bisphosphate carboxylase (RuBPCase) from barley (Hordeum vulgare var Numar) leaves was investigated. RuBPCase purified by chromatography on agarose gel and diethylaminoethyl-cellulose was free of associated endoproteolytic activity. The addition of leupeptin and casein to the extraction buffer completely eliminated proteolysis during initial extraction of RuBPCase. Endoproteolytic activity previously found associated with RuBPCase was identified as being due to contamination of endoproteinases from broken vacuoles.

摘要

研究了与纯化的大麦（Hordeum vulgare var Numar）叶片中的核酮糖二磷酸羧化酶（RuBPCase）相关的内切蛋白酶活性。通过琼脂糖凝胶和二乙基氨基乙基纤维素层析纯化的 RuBPCase 无相关的内切蛋白酶活性。在最初提取 RuBPCase 时，向提取缓冲液中添加亮抑酶肽和酪蛋白可完全消除蛋白水解。先前与 RuBPCase 相关的内切蛋白酶活性被鉴定为来自破裂液泡的内肽酶的污染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/12ff8f9434cc/plntphys00574-0086-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/f35eadd09dfc/plntphys00574-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/e2e8b85bda62/plntphys00574-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/12ff8f9434cc/plntphys00574-0086-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/f35eadd09dfc/plntphys00574-0085-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/e2e8b85bda62/plntphys00574-0086-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a76c/1066837/12ff8f9434cc/plntphys00574-0086-b.jpg

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本文引用的文献

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2
Intracellular Localization of Peptide Hydrolases in Wheat (Triticum aestivum L.) Leaves.小麦叶片中肽水解酶的细胞内定位。
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3
Vacuolar localization of proteases and degradation of chloroplasts in mesophyll protoplasts from senescing primary wheat leaves.液泡定位蛋白酶和叶绿体在衰老小麦叶片原生质体中的降解。
乙烯利对番茄叶片蛋白降解及病程相关蛋白积累的影响。
Plant Physiol. 1990 Jan;92(1):227-33. doi: 10.1104/pp.92.1.227.
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Pathogenesis-related proteins of tomato : p-69 as an alkaline endoproteinase.番茄病程相关蛋白：p-69 作为一种碱性内切蛋白酶。
Plant Physiol. 1988 May;87(1):58-63. doi: 10.1104/pp.87.1.58.
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Neutral peptidases in the stroma of pea chloroplasts.豌豆叶绿体基质中的中性肽酶。
Plant Physiol. 1986 Jun;81(2):603-8. doi: 10.1104/pp.81.2.603.
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7
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