Thom M, Maretzki A
Experiment Station. Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701.
Plant Physiol. 1985 Apr;77(4):873-6. doi: 10.1104/pp.77.4.873.
A plasmalemma-bound NADH-dependent redox system has been identified in protoplasts isolated from cell suspensions of sugarcane. This system oxidized NADH as well as NADPH, increased O(2) consumption 3-fold, and increased the pH of the external medium while the cytoplasmic pH was decreased. In the presence of NADH, ferricyanide was rapidly reduced and the external medium was acidified. The uptake rates of K(+), 3-O-methylglucose, leucine, and arginine were all decreased in the presence of NADH.
在从甘蔗细胞悬浮液中分离出的原生质体中,已鉴定出一种与质膜结合的依赖于NADH的氧化还原系统。该系统可氧化NADH以及NADPH,使氧气消耗量增加3倍,并使外部介质的pH值升高,同时细胞质pH值降低。在NADH存在的情况下,铁氰化物迅速被还原,外部介质被酸化。在NADH存在的情况下,K(+)、3 - O - 甲基葡萄糖、亮氨酸和精氨酸的摄取率均降低。