Department of Chemistry, Indiana State University, Terre Haute, Indiana 47809.
Plant Physiol. 1986 Jun;81(2):614-8. doi: 10.1104/pp.81.2.614.
Antibodies to broad bean polyphenoloxidase (PPO) were used to detect and demonstrate that the PPOs found in several different plants have many similarities in common. Crude extracts from leaves of broad bean, bush bean, lettuce, mung bean, pea, soybean, spinach, tobacco, and tomato contained enzyme which cross-reacted with polyclonal anti-PPO in Ouchterlony double diffusion analysis. The results suggested that plant polyphenoloxidase from a wide range of species may contain similar antigen determinants. Poly A(+) mRNA was isolated from leaves of each plant species and translated in vitro using a rabbit reticulocyte translation system. An in vitro synthesized product corresponding to PPO from each species was identified after specific immunoprecipitation with anti-PPO. The molecular weight of this in vitro product was similar in all plants examined and found to be approximately 45 kilodaltons. Peptide maps of the in vitro synthesized product from all plant species were similar and showed at least three peptides in common. Plant PPOs may have more structural similarities than commonly though in spite of the great variety in observed isoenzyme forms.
抗菜豆多酚氧化酶（PPO）抗体被用来检测和证明几种不同植物中的 PPO 具有许多共同的相似之处。粗提自菜豆、矮生菜豆、生菜、绿豆、豌豆、大豆、菠菜、烟草和番茄叶片中的酶，在奥克特罗尼双扩散分析中与多克隆抗 PPO 发生交叉反应。结果表明，来自广泛物种的植物多酚氧化酶可能含有相似的抗原决定簇。从每种植物的叶片中分离出多 A(+)mRNA，并使用兔网织红细胞翻译系统进行体外翻译。用抗 PPO 特异性免疫沉淀后，鉴定出与每种物种的 PPO 相对应的体外合成产物。该体外产物的分子量在所有被检测的植物中相似，约为 45 千道尔顿。所有植物物种的体外合成产物的肽图谱相似，至少有三个共同的肽。尽管观察到同工酶形式的多样性很大，但植物 PPO 可能具有比通常认为更多的结构相似性。
