一种测定脱落酸的高灵敏度定量方法。
A highly sensitive method for quantitative determination of abscisic Acid.
机构信息
Department of Horticulture, The Ohio Agricultural Research and Development Center, The Ohio State University, Columbus, Ohio 43210.
出版信息
Plant Physiol. 1986 Oct;82(2):600-3. doi: 10.1104/pp.82.2.600.
Abstract
An abscisic acid derivative was formed by reaction with pentafluorobenzyl bromide which allowed highly sensitive detection by gas-liquid chromatography with electron capture detection. In comparison to the methyl ester derivative, the pentafluorobenzyl derivative of abscisic acid was four times more sensitive to electron capture detection and was stable at room temperature in the presence of ultraviolet light. Derivatization was rapid and the molecular weight of the new compound was confirmed by gas-liquid chromatography-mass spectrometry.
摘要
一种脱落酸衍生物通过与五氟苄基溴反应形成，这使得通过带有电子捕获检测的气液色谱法进行高度灵敏的检测成为可能。与甲酯衍生物相比，脱落酸的五氟苄基衍生物对电子捕获检测的灵敏度提高了四倍，并且在存在紫外光的情况下在室温下稳定。衍生化过程迅速，新化合物的分子量通过气相色谱-质谱法得到确认。
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