Funada R, Sugiyama T, Kubo T, Fushitani M
Department of Forest Products, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan.
Plant Physiol. 1988 Nov;88(3):525-7. doi: 10.1104/pp.88.3.525.
Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectivity for ABA determination. ABA concentrations in cambial region scrapings were independent of the cessation of cambial activity.
以六氘代脱落酸为内标,采用气相色谱-质谱选择离子监测法对赤松(Pinus densiflora)茎中的脱落酸(ABA)进行了鉴定和定量。使用叔丁基二甲基甲硅烷基酯作为ABA的衍生物。该衍生物对ABA测定具有高灵敏度和选择性。形成层区域刮屑中的ABA浓度与形成层活动的停止无关。