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采用选择离子监测法测定赤松中脱落酸的含量。

Determination of Abscisic Acid in Pinus densiflora by Selected Ion Monitoring.

作者信息

Funada R, Sugiyama T, Kubo T, Fushitani M

机构信息

Department of Forest Products, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183, Japan.

出版信息

Plant Physiol. 1988 Nov;88(3):525-7. doi: 10.1104/pp.88.3.525.

DOI:10.1104/pp.88.3.525
PMID:16666340
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1055616/
Abstract

Abscisic acid (ABA) in the stem of akamatsu (Pinus densiflora) was identified and quantified by gas chromatography-mass spectrometry-selected ion monitoring using hexadeuterated ABA as an internal standard. tert-Butyldimethylsilyl ester was used as a derivative of ABA. This derivative had high sensitivity and selectivity for ABA determination. ABA concentrations in cambial region scrapings were independent of the cessation of cambial activity.

摘要

以六氘代脱落酸为内标,采用气相色谱-质谱选择离子监测法对赤松(Pinus densiflora)茎中的脱落酸(ABA)进行了鉴定和定量。使用叔丁基二甲基甲硅烷基酯作为ABA的衍生物。该衍生物对ABA测定具有高灵敏度和选择性。形成层区域刮屑中的ABA浓度与形成层活动的停止无关。

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本文引用的文献

1
A highly sensitive method for quantitative determination of abscisic Acid.一种测定脱落酸的高灵敏度定量方法。
Plant Physiol. 1986 Oct;82(2):600-3. doi: 10.1104/pp.82.2.600.
2
Trace analysis by gas chromatography/mass spectrometry (GC/MS) using a tandem double-focusing/quadrupole instrument.
Biomed Mass Spectrom. 1985 Mar;12(3):139-41. doi: 10.1002/bms.1200120309.
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Dual metastable peak monitoring: application to the analysis of oestradiol-17 beta as the bis (tert-butyldimethylsilyl) ether.双亚稳峰监测:应用于分析作为双(叔丁基二甲基甲硅烷基)醚的雌二醇-17β。
Biomed Mass Spectrom. 1985 Jan;12(1):19-24. doi: 10.1002/bms.1200120105.
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The determination of terbutaline in human plasma by selected ion monitoring of the t-butyldimethylsilyl ether.
Biomed Mass Spectrom. 1979 Oct;6(10):460-1. doi: 10.1002/bms.1200061011.
5
Tert-butyl dimethylsilyl ethers as derivatives for qualitative analysis of steroids and prostaglandins by gas phase methods.
Anal Chem. 1976 Mar;48(3):465-7. doi: 10.1021/ac60367a001.