Department of Biology and Molecular Biology Institute, University of California, Los Angeles, California 90024.
Plant Physiol. 1986 Dec;82(4):1172-4. doi: 10.1104/pp.82.4.1172.
We have previously isolated and sequenced two genes encoding light-harvesting chlorophyll a/b-proteins (LHCP) from Lemna gibba. One of these, AB30, encodes a protein that is highly homologous to LHCP sequences reported from other species, but the second, AB19, encodes a protein that has a transit peptide and first 12 amino-terminal residues of the mature protein that are substantially different. Despite these differences, we can demonstrate that AB19 encoded protein synthesized in vitro can be imported into isolated chloroplasts, and we provide evidence that at least some of the imported molecules are assembled into the light-harvesting complex of photosystem II. Thus, our results are consistent with the possibility that there are two functional forms of LHCP.
我们之前从浮萍（Lemna gibba）中分离并测序了两个编码叶绿素 a/b 光捕获蛋白（LHCP）的基因。其中一个，AB30，编码的蛋白质与其他物种报道的 LHCP 序列高度同源，但第二个，AB19，编码的蛋白质具有转运肽和成熟蛋白质的前 12 个氨基酸残基，有很大的不同。尽管存在这些差异，我们可以证明体外合成的 AB19 编码蛋白可以被导入到分离的叶绿体中，并且我们提供了证据表明至少一些导入的分子被组装到光系统 II 的光捕获复合物中。因此，我们的结果与存在两种功能性 LHCP 形式的可能性一致。
