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蓖麻叶和胚乳组织中 ATP 依赖性果糖 6-磷酸 1-磷酸转移酶同工酶的特性。

Characterization of ATP-Dependent Fructose 6-Phosphate 1-Phosphotransferase Isozymes from Leaf and Endosperm Tissues of Ricinus communis.

机构信息

Department of Biology, Queen's University, Kingston, Ontario K7L 3N6 Canada.

出版信息

Plant Physiol. 1990 Jan;92(1):155-9. doi: 10.1104/pp.92.1.155.

DOI:10.1104/pp.92.1.155
PMID:16667239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1062263/
Abstract

Plastid and cytosolic isozymes of ATP:fructose 6-phosphate 1-phosphotransferase (PFK(p) and PFK(c), respectively) have been isolated from leaves and developing endosperm tissues of the castor oil plant (Ricinus communis L). Endosperm PFK(p) has been purified to apparent homogeneity. Polyclonal antibodies raised against one of the four polypeptides associated with potato tuber PFK (molecular mass, 46 kilodaltons) immunoprecipitated developing endosperm and leaf PFK(p), but not PFK(c) isozymes. Western blots, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and analytical gel filtration show that PFK(p) from developing endosperm is a 220 kilodalton homotetramer composed of 57 kilodalton subunits. Kinetic studies of leaf PFK(p) and PFK(c) isozymes reveal both similarities and differences to the characteristics of the respective endosperm isozymes studied previously (WJ Garland, DT Dennis [1980] Arch Biochem Biophys 204: 302-317). The immunological and kinetic data suggest that leaf and developing endosperm PFK(p) are different but structurally related proteins.

摘要

质体和胞质同工型的 ATP:果糖-6-磷酸 1-磷酸转移酶（PFK(p) 和 PFK(c)）已从蓖麻植物（Ricinus communis L.）的叶片和发育胚乳组织中分离出来。胚乳 PFK(p) 已被纯化至明显的均一性。针对与马铃薯块茎 PFK 相关的四个多肽之一（分子量为 46 千道尔顿）产生的多克隆抗体免疫沉淀了发育中的胚乳和叶片 PFK(p)，但不沉淀 PFK(c)同工型。Western blot、十二烷基硫酸钠聚丙烯酰胺凝胶电泳和分析凝胶过滤表明，来自发育中的胚乳的 PFK(p) 是由 57 千道尔顿亚基组成的 220 千道尔顿同源四聚体。对叶片 PFK(p) 和 PFK(c)同工型的动力学研究揭示了与先前研究的相应胚乳同工型的特征既有相似之处，也有不同之处（WJ Garland，DT Dennis [1980] Arch Biochem Biophys 204: 302-317）。免疫和动力学数据表明，叶片和发育中的胚乳 PFK(p) 是不同但结构相关的蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a3e/1062263/92cf587f8f38/plntphys00674-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a3e/1062263/92cf587f8f38/plntphys00674-0172-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9a3e/1062263/92cf587f8f38/plntphys00674-0172-a.jpg

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2
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