The Laboratory for Microalgal Biotechnology, Jacob Blaustein Institute for Desert Research, Ben-Gurion University of the Negev, Sede-Boker Campus 84990, Israel.
Plant Physiol. 1992 Feb;98(2):569-72. doi: 10.1104/pp.98.2.569.
The pharmaceutical interest and limited availability of gamma-linolenic acid (GLA) and eicosapentaenoic acid (EPA) prompted the search for genetic means for increasing the production of these fatty acids from algal sources. Cell lines of Spirulina platensis and Porphyridium cruentum resistant to the growth inhibition of the herbicide Sandoz 9785 were selected by serial transfers of the culture in the presence of increasing concentrations of the herbicide. The resistant cell lines of S. platensis overproduced GLA and those of P. cruentum overproduced EPA and were stable for at least 50 generations in the absence of the inhibitor.
药物利益和γ-亚麻酸（GLA）和二十碳五烯酸（EPA）的有限供应促使人们寻找从藻类来源增加这些脂肪酸产量的遗传方法。通过在含有不断增加浓度的除草剂的情况下进行连续传代，选择对除草剂 Sandoz 9785 的生长抑制具有抗性的螺旋藻和血紫球藻细胞系。螺旋藻的抗性细胞系过量产生 GLA，而血紫球藻的抗性细胞系过量产生 EPA，并且在没有抑制剂的情况下至少稳定 50 代。
