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发根农杆菌 Ri 质粒 rolC 基因 5'上游区缺失分析与组织特异性表达有关。

Deletion Analysis of the 5'-Upstream Region of the Agrobacterium rhizogenes Ri Plasmid rolC Gene Required for Tissue-Specific Expression.

机构信息

Graduate School of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305.

出版信息

Plant Physiol. 1992 Jun;99(2):464-7. doi: 10.1104/pp.99.2.464.

DOI:10.1104/pp.99.2.464
PMID:16668908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1080485/
Abstract

The cis-acting elements located in the -848 and +23 region of the 5'-upstream region of the rolC gene of the Agrobacterium rhizogenes Ri plasmid were investigated. The cis-acting DNA region required for phloem-specific expression was found within the -153 region, whereas a minimum region needed for the expression in the seed embryo was located at position -120.

摘要

研究了发根农杆菌 Ri 质粒 rolC 基因 5'-上游区的-848 和+23 处的顺式作用元件。在 -153 区发现了韧皮部特异性表达所需的顺式作用 DNA 区,而在种子胚中表达所需的最小区域则位于 -120 位。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b98b/1080485/a8113f418508/plntphys00706-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b98b/1080485/a8113f418508/plntphys00706-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b98b/1080485/a8113f418508/plntphys00706-0111-a.jpg

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本文引用的文献

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Conditions Favorable for the Somatic Embryogenesis in Carrot Cell Culture Enhance Expression of the roIC Promoter-GUS Fusion Gene.在胡萝卜细胞培养中有利于体细胞胚胎发生的条件增强了 roIC 启动子-GUS 融合基因的表达。
Plant Physiol. 1991 Jan;95(1):238-41. doi: 10.1104/pp.95.1.238.
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Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes.毛状根:质粒编码发根农杆菌中的致病特性。
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Binary Agrobacterium vectors for plant transformation.用于植物转化的二元农杆菌载体。
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4
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Plant Physiol. 1994 Apr;104(4):1151-7. doi: 10.1104/pp.104.4.1151.
5
Expression of Agrobacterium rhizogenes auxin biosynthesis genes in transgenic tobacco plants.发根农杆菌生长素生物合成基因在转基因烟草植株中的表达。
Plant Mol Biol. 1995 Apr;28(1):123-36. doi: 10.1007/BF00042044.
Nucleic Acids Res. 1984 Nov 26;12(22):8711-21. doi: 10.1093/nar/12.22.8711.
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RY repeats are conserved in the 5'-flanking regions of legume seed-protein genes.RY重复序列在豆科植物种子蛋白基因的5'侧翼区域中是保守的。
Nucleic Acids Res. 1988 Jan 11;16(1):371. doi: 10.1093/nar/16.1.371.
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GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants.GUS融合:β-葡萄糖醛酸酶作为高等植物中一种灵敏且通用的基因融合标记
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DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
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