Department of Botany, Iowa State University, Ames, Iowa 50011.
Plant Physiol. 1992 Aug;99(4):1699-703. doi: 10.1104/pp.99.4.1699.
The activities of four biotin enzymes, acetyl-coenzyme A (CoA) carboxylase, 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase, and the accumulation of six biotin-containing polypeptides were determined during development of somatic embryos of carrot (Daucus carota). Acetyl-CoA carboxylase activity increased more than sevenfold, whereas the activities of 3-methylcrotonyl-CoA carboxylase, pyruvate carboxylase, and propionyl-CoA carboxylase were relatively unaltered. An increase also occurred in the accumulation of three of the biotin-containing polypeptides (molecular masses of 220, 62, and 34 kilodaltons). Of these, the most dramatic change was in the accumulation of the 62-kilodalton biotin-containing polypeptide, which increased by at least 50-fold as embryogenic cell clusters developed into torpedo embryos.
在胡萝卜体细胞胚发育过程中，测定了四种生物素酶（乙酰辅酶 A（CoA）羧化酶、3-甲基戊烯酰辅酶 A 羧化酶、丙酮酸羧化酶和丙酰辅酶 A 羧化酶）的活性和六种含生物素多肽的积累。乙酰辅酶 A 羧化酶活性增加了 7 倍以上，而 3-甲基戊烯酰辅酶 A 羧化酶、丙酮酸羧化酶和丙酰辅酶 A 羧化酶的活性相对不变。三种含生物素多肽（分子量为 220、62 和 34 千道尔顿）的积累也增加了。其中，62 千道尔顿含生物素多肽的积累变化最为显著，随着胚性细胞团发育成鱼雷胚，其积累增加了至少 50 倍。
