Wu Yihang, Zhou Changxin, Li Xiangping, Song Liyan, Wu Xiumei, Lin Wenyan, Chen Haiyong, Bai Hua, Zhao Jun, Zhang Rongping, Sun Handong, Zhao Yu
Department of Traditional Chinese Medicine and Natural Drug Research, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310031, China.
Phytother Res. 2006 Jul;20(7):585-90. doi: 10.1002/ptr.1918.
The antiinflammatory effect of the total flavonoids of Laggera pterodonta (TFLP) was evaluated with various in vivo models of both acute and chronic inflammation. In the acute inflammation tests, TFLP significantly inhibited xylene-induced mouse ear oedema, carrageenan-induced rat paw oedema and acetic acid-induced mouse vascular permeability. In the carrageenan-induced rat pleurisy model, TFLP efficiently suppressed inflammatory exudate and leukocyte migration, reduced the serum levels of lysozyme (LZM) and malondialdehyde (MDA), increased the activity of serum superoxide dismutase (SOD), and also decreased the contents of total protein, nitric oxide (NO) and prostaglandin E2 (PGE2) in the pleural exudates. No marked effect of TFLP on the activity of serum glutathione peroxidase (GSH-PX) was observed. In the chronic inflammation experiment, TFLP inhibited cotton pellet-induced rat granuloma. The antiinflammatory mechanisms of TFLP are probably associated with the inhibition of prostaglandin formation, influence on the antioxidant systems and the suppression of LZM release. The acute toxicity study revealed that TFLP was nontoxic in mice up to an oral dose of 7.5 g/kg body weight.
采用多种急慢性炎症的体内模型对翼齿六棱菊总黄酮（TFLP）的抗炎作用进行了评价。在急性炎症试验中，TFLP显著抑制二甲苯所致的小鼠耳肿胀、角叉菜胶所致的大鼠足肿胀以及醋酸所致的小鼠血管通透性。在角叉菜胶所致的大鼠胸膜炎模型中，TFLP有效抑制炎症渗出和白细胞迁移，降低血清溶菌酶（LZM）和丙二醛（MDA）水平，提高血清超氧化物歧化酶（SOD）活性，同时降低胸腔渗出液中总蛋白、一氧化氮（NO）和前列腺素E2（PGE2）的含量。未观察到TFLP对血清谷胱甘肽过氧化物酶（GSH-PX）活性有明显影响。在慢性炎症实验中，TFLP抑制棉球所致的大鼠肉芽肿。TFLP的抗炎机制可能与抑制前列腺素形成、影响抗氧化系统以及抑制LZM释放有关。急性毒性研究表明，口服剂量高达7.5 g/kg体重时，TFLP对小鼠无毒。
