Bioengineering Division, Institute of Science, Hacettepe University, Ankara, Turkey.
Medical Biology Division, Faculty of Medicine, Kirsehir Ahi Evran University, Kirsehir, Turkey.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110293. doi: 10.1016/j.msec.2019.110293. Epub 2019 Oct 8.
The inadequate donor source and the difficulty of using natural grafts in tendon repair and regeneration has led researchers to develop biodegradable and biocompatible synthetic based tissue equivalents. Poly(glycerol sebacate) (PGS) is a surface-erodible bioelastomer and has been increasingly investigated in a variety of biomedical applications. In this study, PGS elastomeric sheets were prepared by using a facile microwave method and used as elastomeric platform for the first time under mechanical stimulation to induct the tenocyte gene expression. It is revealed that elastomeric PGS sheets promote progenitor tendon cell structure by increasing proliferation and gene expression with regard to tendon extracellular matrix components. Human tenocytes were seeded onto poly(glycerol-sebacate) sheets and were cultured two days prior to transfer to dynamic culture in a bioreactor system. Cell culture studies were carried out for 12 days under 0%, 3% and 6% strain at 0.33 Hz. The PGS-cell constructs were examined by using Scanning Electron Microscopy (SEM), cell viability via live/dead staining using confocal microscopy, and GAG/DNA analysis. In addition, gene expression was examined using real-time polymerase chain reaction (RT-PCR). Tenocytes cultured upon PGS scaffolds under 6% cyclic strain exhibited tendon-like gene expression profile compared to 3% and 0% strain groups. The results of this study show that PGS is a suitable material in promoting tendon tissue formation under dynamic conditions.
由于供体来源不足以及在肌腱修复和再生中使用天然移植物的困难，研究人员开发了可生物降解和生物相容的合成组织等效物。聚（癸二酸甘油酯）（PGS）是一种表面可侵蚀的生物弹性体，在各种生物医学应用中得到了越来越多的研究。在这项研究中，通过使用简便的微波方法制备了 PGS 弹性体薄片，并首次在机械刺激下用作弹性体平台，以诱导肌腱细胞基因表达。结果表明，弹性 PGS 薄片通过增加增殖和肌腱细胞外基质成分的基因表达来促进前体细胞的结构。将人肌腱细胞接种到聚（癸二酸甘油酯）薄片上，并在转移到生物反应器系统中的动态培养之前培养两天。在 0.33Hz 下以 0%、3%和 6%的应变进行为期 12 天的细胞培养研究。通过扫描电子显微镜（SEM）、使用共聚焦显微镜进行活/死染色的细胞活力以及 GAG/DNA 分析来检查 PGS-细胞构建体。此外，通过实时聚合酶链反应（RT-PCR）检查基因表达。与 3%和 0%应变组相比，在 PGS 支架上培养的肌腱细胞在 6%循环应变下表现出肌腱样基因表达谱。这项研究的结果表明，PGS 是在动态条件下促进肌腱组织形成的合适材料。
