Scholler Nathalie, Garvik Barbara, Hayden-Ledbetter Martha, Kline Toni, Urban Nicole
Molecular Diagnostics Program, Public Health Sciences, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, WA 98109-1024, USA.
Cancer Lett. 2007 Mar 8;247(1):130-6. doi: 10.1016/j.canlet.2006.03.029. Epub 2006 May 4.
Preventing peritoneal implantation of ovarian carcinoma cells could prolong patient remission and survival. CA125 is expressed on most ovarian cancer cells and was reported to be a ligand of mesothelin, a peritoneal protein. We developed a cell adhesion assay with CA125-expresser ovarian cancer cells and human mesothelin-transfected cells and we confirmed that CA125 and mesothelin mediate cell attachment. We also showed that this assay supplies a high-throughput screening system for reagents able to block CA125/mesothelin-dependent cell attachment with a sensitive quantitative readout. We finally demonstrated that a mesothelin chimeric protein and anti-CA125 antibodies block CA125/mesothelin-dependent cell attachment.
预防卵巢癌细胞的腹膜种植可延长患者的缓解期和生存期。大多数卵巢癌细胞都表达CA125，据报道它是一种腹膜蛋白间皮素的配体。我们利用表达CA125的卵巢癌细胞和转染了人源间皮素的细胞开发了一种细胞黏附试验，并证实CA125和间皮素介导细胞黏附。我们还表明，该试验为能够阻断CA125/间皮素依赖性细胞黏附的试剂提供了一个高通量筛选系统，并具有灵敏的定量读数。我们最终证明，一种间皮素嵌合蛋白和抗CA125抗体可阻断CA125/间皮素依赖性细胞黏附。
