用于快速检测动物源大肠杆菌中β-内酰胺酶基因的改进型多重PCR方法
Improved multiplex PCR method for the rapid detection of beta-lactamase genes in Escherichia coli of animal origin.
作者信息
Pomba Constança, Mendonça Nuno, Costa Marta, Louro Deolinda, Baptista Bruno, Ferreira Mónica, Correia José Duarte, Caniça Manuela
机构信息
CIISA, Faculty of Veterinary Medicine, Universidade Técnica de Lisboa, Av. da Universidade Técnica, 1300-477 Lisboa, Portugal.
出版信息
Diagn Microbiol Infect Dis. 2006 Sep;56(1):103-6. doi: 10.1016/j.diagmicrobio.2006.03.005. Epub 2006 May 5.
We developed 2 variants, A and B, of a multiplex polymerase chain reaction method for detecting the beta-lactam resistance genes bla(TEM), bla(SHV), and bla(OXA) in 122 uropathogenic Escherichia coli animal strains. Method B yielded 98% specificity and 100% sensitivity, and method A yielded 100% and 89%, respectively. Variant B was more accurate (99%) than A (94%).
我们开发了两种多重聚合酶链反应方法的变体，即A和B，用于检测122株动物源致病性大肠杆菌中的β-内酰胺抗性基因bla(TEM)、bla(SHV)和bla(OXA)。方法B的特异性为98%，灵敏度为100%，方法A的特异性和灵敏度分别为100%和89%。变体B（99%）比变体A（94%）更准确。
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