Department of Pathology, Laboratory of Immunophysiology, Biological and Health Sciences Center, Federal University of Maranhão (UFMA), 65085-580 São Luis, Brazil.
Evid Based Complement Alternat Med. 2012;2012:157652. doi: 10.1155/2012/157652. Epub 2012 Dec 19.
The immunomodulatory and anti-inflammatory activities of green propolis extracts from Apis mellifera were investigated using acute and chronic inflammation models. Swiss mice were anesthetized and a cotton pellet granuloma was implanted in subcutaneous tissue. Then the mice were divided into six groups and received apyrogenic water or different propolis extracts by oral route (5 mg/kg). According to the treatment the groups were designated as E1A, E1B, E10, E11, and E12. The control group received apyrogenic water. The treatment was performed by six days when the mice were killed. The blood and the bronchoalveolar lavage (BAL) were collected to measure the leukocyte recruitment. In acute pulmonary inflammation, Balb/c mice received lipopolysaccharide (LPS) of Escherichia coli by intranasal route for three days. Concomitantly the mice received by oral route apyrogenic water (control) or E10 and E11 propolis extracts. BAL was performed to assess the inflammatory infiltrate and cytokine quantification. The results showed that the E11 extract has anti-inflammatory property in both models by the inhibition of proinflammatory cytokines and increase of anti-inflammatory cytokines suggesting an immunomodulatory activity.
研究了蜜蜂的绿色蜂胶提取物的免疫调节和抗炎活性，使用急性和慢性炎症模型。将瑞士小鼠麻醉，并将棉绒球植入皮下组织。然后将小鼠分为六组，并通过口服途径（5mg/kg）给予无菌水或不同的蜂胶提取物。根据治疗方法，将组命名为 E1A、E1B、E10、E11 和 E12。对照组给予无菌水。治疗在六天时进行，然后处死小鼠。收集血液和支气管肺泡灌洗液（BAL）以测量白细胞募集。在急性肺炎症中，Balb/c 小鼠通过鼻腔途径接受大肠杆菌脂多糖（LPS）三天。同时，小鼠通过口服途径给予无菌水（对照）或 E10 和 E11 蜂胶提取物。进行 BAL 以评估炎症浸润和细胞因子定量。结果表明，E11 提取物在两种模型中均具有抗炎作用，通过抑制促炎细胞因子和增加抗炎细胞因子来显示免疫调节活性。
