Laboratório de Pesquisa, Desenvolvimento e Inovação (P, D & I)-Apis Flora Industrial e Comercial LTDA, Rua Triunfo, 945, 14020-670 Ribeirão Preto, SP, Brazil ; Departamento de Química, Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes, 3900, 14040-901 Ribeirão Preto, SP, Brazil.
Evid Based Complement Alternat Med. 2013;2013:670451. doi: 10.1155/2013/670451. Epub 2013 Apr 24.
Since the beginning of propolis research, several groups have studied its antibacterial, antifungal, and antiviral properties. However, most of these studies have only employed propolis ethanolic extract (PEE) leading to little knowledge about the biological activities of propolis water extract (PWE). Based on this, in a previous study, we demonstrated the anti-inflammatory and immunomodulatory activities of PWE. In order to better understand the equilibrium between effectiveness and toxicity, which is essential for a new medicine, the characteristics of PWE were analyzed. We developed and validated an RP-HPLC method to chemically characterize PWE and PEE and evaluated the in vitro antioxidant/antimicrobial activity for both extracts and the safety of PWE via determining genotoxic potential using in vitro and in vivo mammalian micronucleus assays. We have concluded that the proposed analytical methodology was reliable, and both extracts showed similar chemical composition. The extracts presented antioxidant and antimicrobial effects, while PWE demonstrated higher antioxidant activity and more efficacious for the most of the microorganisms tested than PEE. Finally, PWE was shown to be safe using micronucleus assays.
自蜂胶研究开始以来，已有几个研究小组研究了其抗菌、抗真菌和抗病毒特性。然而，这些研究大多只采用蜂胶乙醇提取物（PEE），因此对蜂胶水提取物（PWE）的生物活性知之甚少。基于此，在之前的一项研究中，我们证明了 PWE 的抗炎和免疫调节活性。为了更好地理解有效性和毒性之间的平衡，这对于一种新药至关重要，我们分析了 PWE 的特性。我们开发并验证了一种 RP-HPLC 方法来对 PWE 和 PEE 进行化学表征，并评估了两种提取物的体外抗氧化/抗菌活性，以及使用体外和体内哺乳动物微核试验测定遗传毒性潜力来评估 PWE 的安全性。我们得出的结论是，所提出的分析方法可靠，两种提取物具有相似的化学组成。提取物具有抗氧化和抗菌作用，而 PWE 显示出比 PEE 更高的抗氧化活性，对大多数测试的微生物更有效。最后，微核试验表明 PWE 是安全的。
