Irimia Daniel, Geba Dan A, Toner Mehmet
BioMEMS Resource Center, Center for Engineering in Medicine and Surgical Services, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Anal Chem. 2006 May 15;78(10):3472-7. doi: 10.1021/ac0518710.
The study of cellular responses to chemical gradients in vitro would greatly benefit from experimental systems that can generate precise and stable gradients comparable to chemical nonhomogeneities occurring in vivo. Recently, microfluidic devices have been demonstrated for linear gradient generation for biological applications with unmatched accuracy and stability. However, no systematic approach exists at this time for generating other gradients of target spatial configuration. Here we demonstrate experimentally and provide mathematical proof for a systematic approach to generating stable gradients of any profile by the controlled mixing of two starting solutions.
体外细胞对化学梯度反应的研究将极大地受益于能够产生与体内化学非均匀性相当的精确且稳定梯度的实验系统。最近，微流控装置已被证明可用于生物应用中的线性梯度生成，具有无与伦比的准确性和稳定性。然而，目前不存在用于生成其他目标空间配置梯度的系统方法。在此，我们通过实验证明并提供数学证明，表明一种通过两种起始溶液的受控混合来生成任何轮廓稳定梯度的系统方法。
