从埃及三个儿科人群中分离出的福氏志贺氏菌菌株的流行病学及基因特征分析（2000 - 2004年）
Epidemiology and genetic characterization of Shigella flexneri strains isolated from three paediatric populations in Egypt (2000-2004).
作者信息
Ahmed S F, Riddle M S, Wierzba T F, Messih I Abdel, Monteville M R, Sanders J W, Klena J D
机构信息
United States Naval Medical Research Unit - 3, Cairo, Egypt.
出版信息
Epidemiol Infect. 2006 Dec;134(6):1237-48. doi: 10.1017/S095026880600642X. Epub 2006 May 11.
Abstract
Ninety-seven isolates of Shigella flexneri from children seeking medical care from three sites in Egypt were characterized. Overall, 46.4% of children (median age 17 months) were febrile or reported blood in their stools, 25.8% were dehydrated and 16.5% were admitted to hospital. Serotypes 2a (37.1%), 1b (18.6%), 1c (17.5%), and 6 (15.5%) comprised over 88.7% of the total isolates. We observed marked resistance to ampicillin (87.6%), tetracycline (84.5%) and trimethoprim-sulfamethoxazole (63.9%). Pulsed-field electrophoresis grouped the majority of isolates within a serotype together, separately from isolates of an alternative serotype. The set gene was present in all serogroup 2a isolates, however, the sen gene was detected in every isolate. Our results show S. flexneri 1c has emerged as a dominant S. flexneri serotype in Egypt. Development and application of a Shigella vaccine should consider the diversity of Shigella serotypes within a geographical region prior to administration.
摘要
对从埃及三个地点就医儿童中分离出的97株福氏志贺菌进行了特征分析。总体而言，46.4%的儿童（中位年龄17个月）发热或粪便带血，25.8%脱水，16.5%住院。血清型2a（37.1%）、1b（18.6%）、1c（17.5%）和6（15.5%）占分离株总数的88.7%以上。我们观察到对氨苄西林（87.6%）、四环素（84.5%）和甲氧苄啶-磺胺甲恶唑（63.9%）有明显耐药性。脉冲场凝胶电泳将大多数血清型内的分离株聚集在一起，与其他血清型的分离株分开。set基因存在于所有2a血清群分离株中，然而，sen基因在每个分离株中均被检测到。我们的结果表明，福氏志贺菌1c已成为埃及福氏志贺菌的主要血清型。在接种志贺菌疫苗之前，其开发和应用应考虑地理区域内志贺菌血清型的多样性。
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