Phoon Colin Kit Lun
Pediatric Cardiology Program, New York University School of Medicine, New York 10016, USA.
Pediatr Res. 2006 Jul;60(1):14-21. doi: 10.1203/01.pdr.0000219441.28206.79. Epub 2006 May 11.
Progress has been rapid in the elucidation of genes responsible for cardiac development. Strategies to ascertain phenotypes, however, have lagged behind advances in genomics, particularly in the in vivo mouse embryo, considered a model organism for mammalian development, and for human development and disease. Over the past several years, our laboratory and others have pioneered a variety of ultrasound biomicroscopy (UBM)-Doppler approaches to study in vivo development in both normal and mutant mouse embryos. This state-of-the-art review will discuss the development and potential of ultrasound biomicroscopy as a tool for the in vivo imaging and phenotyping of both cardiac and non-cardiac organ systems in the early developing mouse. Broad, long-term research objectives are to define living structure-function relationships during critical periods of mammalian morphogenesis.
在阐明负责心脏发育的基因方面进展迅速。然而，确定表型的策略却落后于基因组学的进展，特别是在体内小鼠胚胎中，小鼠胚胎被视为哺乳动物发育以及人类发育和疾病的模式生物。在过去几年中，我们实验室和其他机构率先采用了多种超声生物显微镜（UBM）-多普勒方法来研究正常和突变小鼠胚胎的体内发育。这篇前沿综述将讨论超声生物显微镜作为一种工具在早期发育小鼠体内对心脏和非心脏器官系统进行成像和表型分析的发展及潜力。广泛的长期研究目标是确定哺乳动物形态发生关键时期的活体结构-功能关系。
