Reese J M, Reissing M, Daniel R W, Shah K V
Infect Immun. 1975 Jun;11(6):1375-81. doi: 10.1128/iai.11.6.1375-1381.1975.
Urine specimens from 23 children and 9 adults who were undergoing treatment for malignancy as well as urines from 40 normal individuals were concentrated and examined for evidence of papovavirus infection. Papovavirus particles were detected in 6 of 64 urines examined by electron microscopy. Three of the particle-positive urines induced BK virus-specific immunofluorescence after inoculation of WI38 cells, and three isolations of BK virus were made by inoculation of urines from virus-excreting patients into Vero cells. BK virus-specific hemagglutination-inhibiting and immunofluorescence neutralizing antibodies were found in a majority of urines from adult patients, in about a fifth of pediatric patients, and less often in normal urines. Urines of virus-excreting patients generally had antibodies. In indirect fluorescent antibody tests, BK virus-specific antibodies of the immunoglobulin G class were found in five urine specimens from patients; immunoglobulin A antibodies were not detected in any urine. These data suggest that activation of BK virus is related to immunosuppression and not to transplantation itself and that the occurrence of virus-specific antibodies in urine may be indicative of virus multiplication in the urinary tract.
对23名正在接受恶性肿瘤治疗的儿童和9名成人的尿液标本，以及40名正常人的尿液进行浓缩，并检查是否有乳头多瘤空泡病毒感染的证据。通过电子显微镜检查64份尿液，在其中6份中检测到乳头多瘤空泡病毒颗粒。接种WI38细胞后，3份病毒颗粒阳性尿液诱导出BK病毒特异性免疫荧光，通过将病毒排泄患者的尿液接种到Vero细胞中，成功分离出3株BK病毒。在大多数成年患者的尿液中、约五分之一的儿科患者尿液中以及正常尿液中较少发现BK病毒特异性血凝抑制和免疫荧光中和抗体。病毒排泄患者的尿液通常含有抗体。在间接荧光抗体试验中，在5份患者尿液标本中发现了免疫球蛋白G类的BK病毒特异性抗体；在任何尿液中均未检测到免疫球蛋白A抗体。这些数据表明，BK病毒的激活与免疫抑制有关，而与移植本身无关，并且尿液中病毒特异性抗体的出现可能表明病毒在尿路中增殖。
