Jung M, Krech U, Price P C, Pyndiah M N
Arch Virol. 1975;47(1):39-46. doi: 10.1007/BF01315591.
Ten renal transplant recipients showing a significant increase in human polyomavirus antibodies, indicative of an acute infection, were followed up serologically over periods ranging from two months to more than two years. Fifty-four serum specimens were available for the study and they were tested by both haemagglutination-inhibition and complement-fixation. Polyomavirus antigens were prepared from the BK and SV40-like strains of polyomaviruses, and from the SV40 virus. One strain of polyomavirus, related to the BK strain was isolated from the urine of one of these patients. Two other BK strains were recovered from the urine and kidney, respectively, of transplant recipients not included in this study. Sera of these two patients were not obtained until the transplantation was made; they were already highly positive for polyomavirus antibodies, precluding the demonstration of an increase in antibody titer. Serologic results have shown that HAI antibodies persist at high titers throughout the observation period. This persistence ranged from two to four months (four cases), seven to eleven months (three cases) and thirteen to twenty months (three cases). In none of the cases could a decrease of high titer be demonstrated. Moreover, density gradient studies have shown that specific IgM antibodies also tend to persist over many months. Similar serologic results were obtained in complement-fixation tests with a BK antigen. Titers were at least 1 in 30 in the study group, but were not observed among healthy blood donors. All sera were uniformly negative for SV40 and SV40-like antigens. One polyomavirus isolation was successful from urine obtained six months after initial serologic evidence for a polyomavirus infection. The other two viruses were isolated from materials taken four and seven months after first detection of polyomavirus antibodies at high titer. Both serologic evidence and viral isolations seem to indicate that polyomaviruses (BK type) might cause a chronic infection in humans.
十名肾移植受者体内人多瘤病毒抗体显著增加，提示急性感染，对其进行了为期两个月至两年多的血清学随访。本研究共获得54份血清标本，采用血凝抑制试验和补体结合试验进行检测。多瘤病毒抗原由BK和SV40样多瘤病毒株以及SV40病毒制备。从其中一名患者的尿液中分离出一株与BK株相关的多瘤病毒。另外两株BK株分别从本研究未纳入的移植受者的尿液和肾脏中分离得到。这两名患者的血清直到移植后才获得；他们的多瘤病毒抗体已经呈高阳性，无法证明抗体滴度增加。血清学结果显示，血凝抑制抗体在整个观察期内一直保持高滴度。这种持续时间为两到四个月（4例）、七到十一个月（3例）和十三到二十个月（3例）。在所有病例中均未发现高滴度下降。此外，密度梯度研究表明，特异性IgM抗体也倾向于持续存在数月。用BK抗原进行补体结合试验也得到了类似的血清学结果。研究组的滴度至少为1:30，但在健康献血者中未观察到。所有血清对SV40和SV40样抗原均呈一致阴性。在首次出现多瘤病毒感染血清学证据六个月后，从尿液中成功分离出一株多瘤病毒。另外两株病毒是在首次检测到多瘤病毒抗体高滴度后四个月和七个月从取材中分离得到的。血清学证据和病毒分离似乎都表明，多瘤病毒（BK型）可能会引起人类慢性感染。
