Rees R C, Bray J, Robins R A, Baldwin R W
Int J Cancer. 1975 May 15;15(5):762-72. doi: 10.1002/ijc.2910150507.
Lymph-node cells (LNC) from multiparous pregnant rats were separated on columns prepared from nylon wool, and tested for cytotoxicity against target tumour cells. Reactivity of LNC towards hepatoma D23 and mammary carcinoma AAF57 was demonstrated in cell populations retained on the nylon wool, and not with cells eluted from the column. Although only 25% of the samples of unfractionated LNC were cytotoxic for tumour cells, retained cell fractions were cytotoxic in 11 out of 12 tests (p = less than 0.05). Similarly retained LNC were also cytotoxic for 15-day-old embryo cells but not for normal adult rat fibroblasts. Using multiparous rat serum it was shown that the reactivity of the retained LNC population could be abrogated in eight out of 11 tests (p = less than 0.05). The LNC population recovered from the nylon wool constituted 28 to 35% of the original LNC preparation, and consisted of 60-70% Ig-bearing cells together with a subpopulation of cells responding to soluble PHA. Separation of multiparous LNC on glass beads coated with rat Ig and then rabbit anti-rat Ig (in excess) also demonstrated the retained cell population to be cytotoxic against tumour cells. Approximately 17-20% of the original cell population was recovered from cells retained on the column, and consisted of an enriched Ig-bearing cell population (65-80% Ig-bearing cells) and LNC responsive to PHA. Carbonyl iron treatment of multiparous rat LNC was found to remove detectable cytotoxicity from multiparous rat LNC preparations. The cytotoxicity of multiparous rat LNC retained on nylon wool was also abolished following incubation with carbonyl iron. Definite conclusions as to the nature of the effector cell cannot be drawn from this test, since carbonyl iron treatment was found to remove not only phagocytic cells from LNC preparations but also a proportion of other cell populations including Ig-bearing lymphocytes... In addition to detecting a cytotoxic LNC population reactive towards tumour-associated embryonic antigens (retained fractions from nylon-wool column separation), a subpopulation of multiparous rat LNC was demonstrated in cell fractions eluted from the nylon wool which was shown to suppress the cytotoxicity of the retained multiparous LNC population. The exact nature of this subpopulation of LNC and the mechanism of action is at present not known.
从经产怀孕大鼠中分离出的淋巴结细胞（LNC），通过尼龙毛制备的柱进行分离，并检测其对靶肿瘤细胞的细胞毒性。在尼龙毛上保留的细胞群体中，证明了LNC对肝癌D23和乳腺癌AAF57具有反应性，而从柱上洗脱的细胞则没有。尽管未分级的LNC样本中只有25%对肿瘤细胞具有细胞毒性，但保留的细胞组分在12次测试中有11次具有细胞毒性（p<0.05）。同样保留的LNC对15日龄胚胎细胞也具有细胞毒性，但对正常成年大鼠成纤维细胞则没有。使用经产大鼠血清表明，在11次测试中有8次保留的LNC群体的反应性可以被消除（p<0.05）。从尼龙毛中回收的LNC群体占原始LNC制剂的28%至35%，由60 - 70%携带Ig的细胞以及对可溶性PHA有反应的细胞亚群组成。将经产LNC在包被有大鼠Ig然后过量的兔抗大鼠Ig的玻璃珠上进行分离，也证明了保留的细胞群体对肿瘤细胞具有细胞毒性。从柱上保留的细胞中回收了约17 - 20%的原始细胞群体，其由富集的携带Ig的细胞群体（65 - 80%携带Ig的细胞）和对PHA有反应的LNC组成。发现用羰基铁处理经产大鼠LNC可消除经产大鼠LNC制剂中可检测到的细胞毒性。用羰基铁孵育后，保留在尼龙毛上的经产大鼠LNC的细胞毒性也被消除。由于发现羰基铁处理不仅从LNC制剂中去除了吞噬细胞，还去除了包括携带Ig的淋巴细胞在内的一部分其他细胞群体，因此无法从该测试中得出关于效应细胞性质的确切结论……除了检测到对肿瘤相关胚胎抗原有反应的细胞毒性LNC群体（尼龙毛柱分离得到的保留组分）外，在从尼龙毛洗脱的细胞组分中还证明了经产大鼠LNC的一个亚群，该亚群可抑制保留的经产LNC群体的细胞毒性。目前尚不清楚该LNC亚群的确切性质和作用机制。
