1型糖尿病患儿糖尿病视网膜病变发展早期N-乙酰-β-D-氨基葡萄糖苷酶和肿瘤坏死因子-α的活性

The activity of N-acetyl-beta-D-glucosaminidase and tumor necrosis factor-alpha at early stage of diabetic retinopathy development in type 1 diabetes mellitus children.

作者信息

Myśliwiec Małgorzata, Zorena Katarzyna, Balcerska Anna, Myśliwska Jolanta, Lipowski Paweł, Raczyńska Krystyna

机构信息

Institute of Pediatrics, Hematology, Oncology and Endocrinology, Medical University of Gdańsk, ul. Debinki 7, 80-952 Gdańsk, Poland.

出版信息

Clin Biochem. 2006 Aug;39(8):851-6. doi: 10.1016/j.clinbiochem.2006.03.013. Epub 2006 Apr 5.

DOI:10.1016/j.clinbiochem.2006.03.013

PMID:16696964

Abstract

OBJECTIVE

The assessment of the clinical significance of TNF-alpha, IL-10 and NAG with its A and B isoforms concentrations in children with DM type 1 for the detection of early stages of both diabetic retinopathy and nephropathy.

PATIENTS AND METHODS

One hundred and two children with DM type 1 and 35 healthy controls were analyzed. Levels of TNF-alpha, IL10 and total NAG enzyme activity with its A and B isoforms were measured in serum and urine of all participants.

RESULTS

Children with diabetic retinopathy had a significantly higher levels of TNFalpha in serum (P=0.01) in comparison to those without retinopathy. The activity of NAG (P=0.002) and its isoform A (P=0.006) and isoform B (P=0.001) were significantly higher in children with diabetic retinopathy in comparison to those without this complication. Conversely, within the group with retinopathy, more children had detectable concentrations of IL10 in serum as compared to those without retinopathy (P=0.01).

CONCLUSIONS

These results suggest that NAG activity and TNF-alpha concentration in diabetic retinopathy patients might show a relationship with a degree of renal glomeruli epithelial cells and renal proximal tubules damage.

摘要

目的

评估1型糖尿病患儿中肿瘤坏死因子-α（TNF-α）、白细胞介素-10（IL-10）以及N-乙酰-β-D-氨基葡萄糖苷酶（NAG）及其A和B同工型浓度对于检测糖尿病视网膜病变和肾病早期阶段的临床意义。

患者与方法

分析了102例1型糖尿病患儿和35例健康对照者。检测了所有参与者血清和尿液中TNF-α、IL-10水平以及NAG总酶活性及其A和B同工型。

结果

与无视网膜病变的患儿相比，患有糖尿病视网膜病变的患儿血清中TNF-α水平显著更高（P=0.01）。与无该并发症的患儿相比，患有糖尿病视网膜病变的患儿NAG活性（P=0.002）及其同工型A（P=0.006）和同工型B（P=0.001）显著更高。相反，在有视网膜病变的组内，与无视网膜病变的患儿相比，更多患儿血清中可检测到IL-10浓度（P=0.01）。