Gail D B, Massaro G D, Massaro D
J Appl Physiol. 1975 Apr;38(4):623-9. doi: 10.1152/jappl.1975.38.4.623.
We examined the time course of the influence of cycloheximide on descending pressure-volume curves of excised lungs and on protein and lecithin synthesis and oxygen consumption by lung slices. We also looked at the influence of cycloheximide on granular pneumocyte ultrastructure. Excised lungs from cycloheximide-treated animals are more compliant than controls. After ventilation with air, lungs from control and cycloheximide animals show increased retractive forces and a shift to the right of the deflation P-V curve. Incubation at 38 degrees C for 30 min reverses these changes in control lungs, but not in lungs from cycloheximide-treated rabbits. There is no change in liquid delfation P-V curves after cycloheximide. Cycloheximide causes an immediate decrease of 50% in incorporation of radioactive leucine into protein by lung slices. Incorporation of radioactive palmitate into lecithin and oxygen consumption are also decreased by 50% 6 h after cycloheximide. Lamellar bodies in granular pneumocytes are smaller after cycloheximide. Cycloheximide causes a significant increase in the surface density of the lamellar body envelope. Cytoplasmic area of granular pneumocytes is increased after cycloheximide.
我们研究了环己酰亚胺对离体肺脏降压-容量曲线以及肺切片蛋白质和卵磷脂合成及氧消耗的影响的时间进程。我们还观察了环己酰亚胺对颗粒肺细胞超微结构的影响。用环己酰亚胺处理过的动物的离体肺脏比对照更具顺应性。用空气通气后，对照动物和经环己酰亚胺处理的动物的肺脏回缩力增加，放气压力-容量曲线右移。在38℃孵育30分钟可逆转对照肺脏的这些变化，但经环己酰亚胺处理的兔子的肺脏则无此变化。环己酰亚胺处理后液体放气压力-容量曲线无变化。环己酰亚胺可使肺切片中放射性亮氨酸掺入蛋白质的量立即减少50%。环己酰亚胺处理6小时后，放射性棕榈酸掺入卵磷脂的量和氧消耗也减少50%。经环己酰亚胺处理后，颗粒肺细胞中的板层小体变小。环己酰亚胺可使板层小体包膜的表面密度显著增加。经环己酰亚胺处理后，颗粒肺细胞的细胞质面积增加。
