Pillekamp Frank, Reppel Michael, Brockmeier Konrad, Hescheler Juergen
Pediatric Cardiology, University of Cologne, 50931 Cologne, Germany.
J Electrocardiol. 2006 Oct;39(4):425.e1-4. doi: 10.1016/j.jelectrocard.2006.02.008. Epub 2006 May 12.
To evaluate whether field potential recordings from murine ventricular slice preparations serve as a model to investigate impulse propagation.
Late-stage embryonic and neonatal murine hearts were sliced by a vibratome. Slices were placed on planar microelectrode arrays (MEAs). Field potentials of spontaneously beating and electrically stimulated contractions were recorded. The maximal negative deflection of the field potentials (dV / dt) was calculated to assess the local activation time, to create activation sequence maps, and to estimate conduction velocity.
Mapping of impulse propagation of late-stage embryonic and neonatal murine ventricular slices and estimation of conduction velocities is feasible using the MEA technique showing an impulse propagation reflecting anatomical structures and conduction velocities similar to those obtained with other techniques.
The combination of viable ventricular slice preparations with the MEA technique offers a versatile and powerful technique to study cardiac impulse propagation.
评估来自小鼠心室切片标本的场电位记录是否可作为研究冲动传播的模型。
用振动切片机对晚期胚胎和新生小鼠的心脏进行切片。将切片置于平面微电极阵列（MEA）上。记录自发搏动和电刺激收缩的场电位。计算场电位的最大负向偏转（dV/dt）以评估局部激活时间、创建激活序列图并估计传导速度。
使用MEA技术对晚期胚胎和新生小鼠心室切片的冲动传播进行映射并估计传导速度是可行的，显示出的冲动传播反映了解剖结构，且传导速度与用其他技术获得的相似。
活的心室切片标本与MEA技术的结合提供了一种通用且强大的技术来研究心脏冲动传播。
