小鼠乳腺肿瘤样病毒基因序列的存在可能与特定人类乳腺癌的形态有关。
Presence of mouse mammary tumour-like virus gene sequences may be associated with morphology of specific human breast cancer.
作者信息
Lawson J S, Tran D D, Carpenter E, Ford C E, Rawlinson W D, Whitaker N J, Delprado W
机构信息
School of Public Health and Community Medicine, University of New South Wales, Sydney, Australia.
出版信息
J Clin Pathol. 2006 Dec;59(12):1287-92. doi: 10.1136/jcp.2005.035907. Epub 2006 May 12.
BACKGROUND
Mouse mammary tumour virus (MMTV) has a proven role in breast carcinogenesis in wild mice and genetically susceptible in-bred mice. MMTV-like env gene sequences, which indicate the presence of a replication-competent MMTV-like virus, have been identified in some human breast cancers, but rarely in normal breast tissues. However, no evidence for a causal role of an MMTV-like virus in human breast cancer has emerged, although there are precedents for associations between specific histological characteristics of human cancers and the presence of oncogenic viruses.
AIM
To investigate the possibility of an association between breast cancer and MMTV-like viruses.
METHODS
Histological characteristics of invasive ductal human breast cancer specimens were compared with archival MMTV-associated mammary tumours from C3H experimental mice. The presence of MMTV-like env DNA sequences in the human breast cancer specimens was determined by polymerase chain reaction and confirmed by Southern hybridisation.
RESULTS
MMTV-like env gene sequences were identified in 22 of 59 (37.3%) human breast cancer specimens. Seventeen of 43 (39.5%) invasive ductal carcinoma breast cancer specimens and 4 of 16 (25%) ductal carcinoma in situ specimens had some histological characteristics, which were similar to MMTV-associated mouse mammary tumours. However, these similarities were not associated with the presence or absence of MMTV-like gene sequences in the human breast tumour specimens. A significant (p = 0.05) correlation was found between the grade of the human breast cancer and similarity to the mouse mammary tumours. The lower the grade, the greater the similarity.
CONCLUSION
Some human breast cancer specimens, in which MMTV-like env DNA sequences have been identified, were shown to have histological characteristics (morphology) similar to MMTV-associated mouse mammary tumours. These observations are compatible with, but not conclusive of, an association between the presence of MMTV-like env DNA sequences and some human breast cancers.
背景
小鼠乳腺肿瘤病毒（MMTV）在野生小鼠和基因易感的近交系小鼠的乳腺癌发生过程中已被证实具有作用。在一些人类乳腺癌中已鉴定出MMTV样env基因序列，这表明存在具有复制能力的MMTV样病毒，但在正常乳腺组织中很少见。然而，尽管人类癌症的特定组织学特征与致癌病毒的存在之间存在关联的先例，但尚未出现MMTV样病毒在人类乳腺癌中起因果作用的证据。
目的
研究乳腺癌与MMTV样病毒之间存在关联的可能性。
方法
将人类浸润性导管癌标本的组织学特征与来自C3H实验小鼠的存档MMTV相关乳腺肿瘤进行比较。通过聚合酶链反应确定人类乳腺癌标本中MMTV样env DNA序列的存在，并通过Southern杂交进行确认。
结果
在59例人类乳腺癌标本中的22例（37.3%）中鉴定出MMTV样env基因序列。43例浸润性导管癌乳腺癌标本中的17例（39.5%）和16例原位导管癌标本中的4例（25%）具有一些组织学特征，这些特征与MMTV相关的小鼠乳腺肿瘤相似。然而，这些相似性与人类乳腺肿瘤标本中MMTV样基因序列的存在与否无关。发现人类乳腺癌的分级与与小鼠乳腺肿瘤的相似性之间存在显著（p = 0.05）相关性。分级越低，相似性越大。
结论
一些已鉴定出MMTV样env DNA序列的人类乳腺癌标本显示出与MMTV相关的小鼠乳腺肿瘤相似的组织学特征（形态学）。这些观察结果与MMTV样env DNA序列的存在与一些人类乳腺癌之间的关联相符，但尚无定论。
Zotero 插件